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. 1992 Sep 11;20(17):4668. doi: 10.1093/nar/20.17.4668

Methods for improved protein expression using pET vectors.

J Furlong ¹, M Meighan ¹, J Conner ¹, J Murray ¹, J B Clements ¹

PMCID: PMC334207 PMID: 1329026

Abstract

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

Furlong J., Conner J., McLauchlan J., Lankinen H., Galt C., Marsden H. S., Clements J. B. The large subunit of herpes simplex virus type 1 ribonucleotide reductase: expression in Escherichia coli and purification. Virology. 1991 Jun;182(2):846–851. doi: 10.1016/0042-6822(91)90627-n. [DOI] [PubMed] [Google Scholar]
Lankinen H., Telford E., MacDonald D., Marsden H. The unique N-terminal domain of the large subunit of herpes simplex virus ribonucleotide reductase is preferentially sensitive to proteolysis. J Gen Virol. 1989 Dec;70(Pt 12):3159–3169. doi: 10.1099/0022-1317-70-12-3159. [DOI] [PubMed] [Google Scholar]
Looman A. C., Bodlaender J., Comstock L. J., Eaton D., Jhurani P., de Boer H. A., van Knippenberg P. H. Influence of the codon following the AUG initiation codon on the expression of a modified lacZ gene in Escherichia coli. EMBO J. 1987 Aug;6(8):2489–2492. doi: 10.1002/j.1460-2075.1987.tb02530.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
Studier F. W., Rosenberg A. H., Dunn J. J., Dubendorff J. W. Use of T7 RNA polymerase to direct expression of cloned genes. Methods Enzymol. 1990;185:60–89. doi: 10.1016/0076-6879(90)85008-c. [DOI] [PubMed] [Google Scholar]

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