Skip to main content

. 1992 Sep 11;20(17):4671–4672. doi: 10.1093/nar/20.17.4671

A hit-and-run system for targeted genetic manipulations in yeast.

J Roca ¹, M R Gartenberg ¹, Y Oshima ¹, J C Wang ¹

PMCID: PMC334209 PMID: 1408777

Abstract

4671

Images in this article

Image
on p.4672

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

Cregg J. M., Madden K. R. Use of site-specific recombination to regenerate selectable markers. Mol Gen Genet. 1989 Oct;219(1-2):320–323. doi: 10.1007/BF00261194. [DOI] [PubMed] [Google Scholar]
Dale E. C., Ow D. W. Gene transfer with subsequent removal of the selection gene from the host genome. Proc Natl Acad Sci U S A. 1991 Dec 1;88(23):10558–10562. doi: 10.1073/pnas.88.23.10558. [DOI] [PMC free article] [PubMed] [Google Scholar]
Golic K. G., Lindquist S. The FLP recombinase of yeast catalyzes site-specific recombination in the Drosophila genome. Cell. 1989 Nov 3;59(3):499–509. doi: 10.1016/0092-8674(89)90033-0. [DOI] [PubMed] [Google Scholar]
Matsuzaki H., Nakajima R., Nishiyama J., Araki H., Oshima Y. Chromosome engineering in Saccharomyces cerevisiae by using a site-specific recombination system of a yeast plasmid. J Bacteriol. 1990 Feb;172(2):610–618. doi: 10.1128/jb.172.2.610-618.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
Rothstein R. J. One-step gene disruption in yeast. Methods Enzymol. 1983;101:202–211. doi: 10.1016/0076-6879(83)01015-0. [DOI] [PubMed] [Google Scholar]
Sauer B. Functional expression of the cre-lox site-specific recombination system in the yeast Saccharomyces cerevisiae. Mol Cell Biol. 1987 Jun;7(6):2087–2096. doi: 10.1128/mcb.7.6.2087. [DOI] [PMC free article] [PubMed] [Google Scholar]
Southern E. M. Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol. 1975 Nov 5;98(3):503–517. doi: 10.1016/s0022-2836(75)80083-0. [DOI] [PubMed] [Google Scholar]

ACTIONS

RESOURCES