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. 1992 Sep 25;20(18):4747–4751. doi: 10.1093/nar/20.18.4747

Heteronuclear NMR of DNA with the heteronucleus in natural abundance: facilitated assignment and extraction of coupling constants.

P Schmieder 1, J H Ippel 1, H van den Elst 1, G A van der Marel 1, J H van Boom 1, C Altona 1, H Kessler 1
PMCID: PMC334227  PMID: 1408787

Abstract

Two heteronuclear proton-carbon NMR experiments are applied to the DNA-octamer d(TTGGCCAA)2 with carbon in natural abundance. They lead to a complete assignment of the carbon resonances of the sugars and bases. In addition, several heteronuclear coupling constants, proton-carbon as well as proton-phosphorous and phosphorous-carbon, were determined. The information can be obtained in a reasonable measuring time and offers valuable information for a detailed picture of DNA structure.

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Selected References

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  1. Ashcroft J., Live D. H., Patel D. J., Cowburn D. Heteronuclear two-dimensional 15N- and 13C-NMR studies of DNA oligomers and their netropsin complexes using indirect proton detection. Biopolymers. 1991 Jan;31(1):45–55. doi: 10.1002/bip.360310105. [DOI] [PubMed] [Google Scholar]
  2. Bax A., Sparks S. W., Torchia D. A. Detection of insensitive nuclei. Methods Enzymol. 1989;176:134–150. doi: 10.1016/0076-6879(89)76009-2. [DOI] [PubMed] [Google Scholar]
  3. Fesik S. W., Zuiderweg E. R. Heteronuclear three-dimensional NMR spectroscopy of isotopically labelled biological macromolecules. Q Rev Biophys. 1990 May;23(2):97–131. doi: 10.1017/s0033583500005515. [DOI] [PubMed] [Google Scholar]
  4. Ikura M., Kay L. E., Bax A. A novel approach for sequential assignment of 1H, 13C, and 15N spectra of proteins: heteronuclear triple-resonance three-dimensional NMR spectroscopy. Application to calmodulin. Biochemistry. 1990 May 15;29(19):4659–4667. doi: 10.1021/bi00471a022. [DOI] [PubMed] [Google Scholar]
  5. LaPlante S. R., Ashcroft J., Cowburn D., Levy G. C., Borer P. N. 13C NMR assignments of the protonated carbons of [d(TAGCGCTA)]2 by two-dimensional proton-detected heteronuclear correlation. J Biomol Struct Dyn. 1988 Apr;5(5):1089–1099. doi: 10.1080/07391102.1988.10506450. [DOI] [PubMed] [Google Scholar]
  6. LaPlante S. R., Boudreau E. A., Zanatta N., Levy G. C., Borer P. N., Ashcroft J., Cowburn D. 13C NMR of the bases of three DNA oligonucleotide duplexes: assignment methods and structural features. Biochemistry. 1988 Oct 4;27(20):7902–7909. doi: 10.1021/bi00420a048. [DOI] [PubMed] [Google Scholar]
  7. Lankhorst P. P., Erkelens C., Haasnoot C. A., Altona C. Carbon-13 NMR in conformational analysis of nucleic acid fragments. Heteronuclear chemical shift correlation spectroscopy of RNA constituents. Nucleic Acids Res. 1983 Oct 25;11(20):7215–7230. doi: 10.1093/nar/11.20.7215. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Leupin W., Wagner G., Denny W. A., Wüthrich K. Assignment of the 13C nuclear magnetic resonance spectrum of a short DNA-duplex with 1H-detected two-dimensional heteronuclear correlation spectroscopy. Nucleic Acids Res. 1987 Jan 12;15(1):267–275. doi: 10.1093/nar/15.1.267. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. McIntosh L. P., Dahlquist F. W. Biosynthetic incorporation of 15N and 13C for assignment and interpretation of nuclear magnetic resonance spectra of proteins. Q Rev Biophys. 1990 Feb;23(1):1–38. doi: 10.1017/s0033583500005400. [DOI] [PubMed] [Google Scholar]
  10. Nikonowicz E. P., Pardi A. Three-dimensional heteronuclear NMR studies of RNA. Nature. 1992 Jan 9;355(6356):184–186. doi: 10.1038/355184a0. [DOI] [PubMed] [Google Scholar]
  11. Santos R. A., Tang P., Harbison G. S. Determination of the DNA sugar pucker using 13C NMR spectroscopy. Biochemistry. 1989 Nov 28;28(24):9372–9378. doi: 10.1021/bi00450a018. [DOI] [PubMed] [Google Scholar]
  12. Schmieder P., Kurz M., Kessler H. Determination of heteronuclear long-range couplings to heteronuclei in natural abundance by two- and three-dimensional NMR spectroscopy. J Biomol NMR. 1991 Nov;1(4):403–420. doi: 10.1007/BF02192863. [DOI] [PubMed] [Google Scholar]
  13. Stockman B. J., Reily M. D., Westler W. M., Ulrich E. L., Markley J. L. Concerted two-dimensional NMR approaches to hydrogen-1, carbon-13, and nitrogen-15 resonance assignments in proteins. Biochemistry. 1989 Jan 10;28(1):230–236. doi: 10.1021/bi00427a032. [DOI] [PubMed] [Google Scholar]
  14. Wagner G. Heteronuclear nuclear magnetic resonance experiments for studies of protein conformation. Methods Enzymol. 1989;176:93–113. doi: 10.1016/0076-6879(89)76007-9. [DOI] [PubMed] [Google Scholar]

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