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. 1992 Sep 25;20(18):4789–4793. doi: 10.1093/nar/20.18.4789

Repair of UV-induced (6-4)photoproducts measured in individual genes in the Drosophila embryonic Kc cell line.

J G de Cock 1, A van Hoffen 1, J Wijnands 1, G Molenaar 1, P H Lohman 1, J C Eeken 1
PMCID: PMC334233  PMID: 1408792

Abstract

The nucleotide excision repair (NER; dark-repair) of (6-4)photoproducts ((6-4)PPs) was assayed in cells from a permanent Drosophila melanogaster embryonic cell line, Kc, after exposure to 20 or 40 J/m2 ultraviolet (UV) light. Induction rates in the transcriptionally active genes Gart and Notch as well as in the inactive white locus is similar. They are formed with a frequency of about one-third of that of cyclobutane pyrimidine dimers (CPDs). In all three genes, (6-4)PPs are repaired with the same rate and to the same extent: 31% of the (6-4)PPs are removed in 4 hours post-irradiation and after 16 hours repair is nearly complete. In none of the three genes strand-specific repair was found. Exposure of cells that were irradiated with 40 J/m2 UV to photoreactivating light for 1 hour prior to dark-repair incubation, resulted in enhanced repair of (6-4)PPs.

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Selected References

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