Abstract
Copy-DNA clones covering the complete coding sequence of human Elongation Factor-1 gamma mRNA have been isolated and characterized. The expression of Elongation Factor-1 in a variety of cell lines and a number of tissues shows a large increase in Elongation Factor-1 mRNA going from tissue to cultured cells (20-fold). Messenger-RNA levels for Elongation Factor-1 alpha, -1 beta and -1 gamma increase in parallel suggesting coordinate regulation of the expression of these genes. Oncogenic transformation in vitro does not strongly affect Elongation Factor-1 mRNA levels.
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- Auffray C., Rougeon F. Purification of mouse immunoglobulin heavy-chain messenger RNAs from total myeloma tumor RNA. Eur J Biochem. 1980 Jun;107(2):303–314. doi: 10.1111/j.1432-1033.1980.tb06030.x. [DOI] [PubMed] [Google Scholar]
- Bellé R., Derancourt J., Poulhe R., Capony J. P., Ozon R., Mulner-Lorillon O. A purified complex from Xenopus oocytes contains a p47 protein, an in vivo substrate of MPF, and a p30 protein respectively homologous to elongation factors EF-1 gamma and EF-1 beta. FEBS Lett. 1989 Sep 11;255(1):101–104. doi: 10.1016/0014-5793(89)81069-5. [DOI] [PubMed] [Google Scholar]
- Brands J. H., Maassen J. A., van Hemert F. J., Amons R., Möller W. The primary structure of the alpha subunit of human elongation factor 1. Structural aspects of guanine-nucleotide-binding sites. Eur J Biochem. 1986 Feb 17;155(1):167–171. doi: 10.1111/j.1432-1033.1986.tb09472.x. [DOI] [PubMed] [Google Scholar]
- Chi K., Jones D. V., Frazier M. L. Expression of an elongation factor 1 gamma-related sequence in adenocarcinomas of the colon. Gastroenterology. 1992 Jul;103(1):98–102. doi: 10.1016/0016-5085(92)91101-9. [DOI] [PubMed] [Google Scholar]
- Cormier P., Osborne H. B., Morales J., Bassez T., Poulhe R., Mazabraud A., Mulner-Lorillon O., Bellé R. Molecular cloning of Xenopus elongation factor 1 gamma, major M-phase promoting factor substrate. Nucleic Acids Res. 1991 Dec 11;19(23):6644–6644. doi: 10.1093/nar/19.23.6644. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Godar D. E., Yang D. C. Mammalian high molecular weight and monomeric forms of valyl-tRNA synthetase. Biochemistry. 1988 Mar 22;27(6):2181–2186. doi: 10.1021/bi00406a055. [DOI] [PubMed] [Google Scholar]
- Grant A. G., Flomen R. M., Tizard M. L., Grant D. A. Differential screening of a human pancreatic adenocarcinoma lambda gt11 expression library has identified increased transcription of elongation factor EF-1 alpha in tumour cells. Int J Cancer. 1992 Mar 12;50(5):740–745. doi: 10.1002/ijc.2910500513. [DOI] [PubMed] [Google Scholar]
- Hsieh S. L., Campbell R. D. Evidence that gene G7a in the human major histocompatibility complex encodes valyl-tRNA synthetase. Biochem J. 1991 Sep 15;278(Pt 3):809–816. doi: 10.1042/bj2780809. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Janssen G. M., Morales J., Schipper A., Labbé J. C., Mulner-Lorillon O., Bellé R., Möller W. A major substrate of maturation promoting factor identified as elongation factor 1 beta gamma delta in Xenopus laevis. J Biol Chem. 1991 Aug 15;266(23):14885–14888. [PubMed] [Google Scholar]
- Janssen G. M., Möller W. Elongation factor 1 beta gamma from Artemia. Purification and properties of its subunits. Eur J Biochem. 1988 Jan 15;171(1-2):119–129. doi: 10.1111/j.1432-1033.1988.tb13766.x. [DOI] [PubMed] [Google Scholar]
- Klein B., Pastink A., Odijk H., Westerveld A., van der Eb A. J. Transformation and immortalization of diploid xeroderma pigmentosum fibroblasts. Exp Cell Res. 1990 Dec;191(2):256–262. doi: 10.1016/0014-4827(90)90012-y. [DOI] [PubMed] [Google Scholar]
- Lew Y., Jones D. V., Mars W. M., Evans D., Byrd D., Frazier M. L. Expression of elongation factor-1 gamma-related sequence in human pancreatic cancer. Pancreas. 1992;7(2):144–152. doi: 10.1097/00006676-199203000-00003. [DOI] [PubMed] [Google Scholar]
- Maessen G. D., Amons R., Zeelen J. P., Möller W. Primary structure of elongation factor 1 gamma from Artemia. FEBS Lett. 1987 Oct 19;223(1):181–186. doi: 10.1016/0014-5793(87)80532-x. [DOI] [PubMed] [Google Scholar]
- Motorin YuA, Wolfson A. D., Orlovsky A. F., Gladilin K. L. Mammalian valyl-tRNA synthetase forms a complex with the first elongation factor. FEBS Lett. 1988 Oct 10;238(2):262–264. doi: 10.1016/0014-5793(88)80492-7. [DOI] [PubMed] [Google Scholar]
- Sanders J., Maassen J. A., Amons R., Möller W. Nucleotide sequence of human elongation factor-1 beta cDNA. Nucleic Acids Res. 1991 Aug 25;19(16):4551–4551. doi: 10.1093/nar/19.16.4551. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Schrier P. I., Bernards R., Vaessen R. T., Houweling A., van der Eb A. J. Expression of class I major histocompatibility antigens switched off by highly oncogenic adenovirus 12 in transformed rat cells. 1983 Oct 27-Nov 2Nature. 305(5937):771–775. doi: 10.1038/305771a0. [DOI] [PubMed] [Google Scholar]
- Timmers H. T., van Zoelen E. J., Bos J. L., van der Eb A. J. Cells transformed by adenovirus type 12 but not by type 5 are dependent on insulin or insulin-like growth factor I for their proliferation. J Biol Chem. 1988 Jan 25;263(3):1329–1335. [PubMed] [Google Scholar]
- van Damme H. T., Amons R., Karssies R., Timmers C. J., Janssen G. M., Möller W. Elongation factor 1 beta of artemia: localization of functional sites and homology to elongation factor 1 delta. Biochim Biophys Acta. 1990 Aug 27;1050(1-3):241–247. doi: 10.1016/0167-4781(90)90174-z. [DOI] [PubMed] [Google Scholar]


