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. 1992 Nov 25;20(22):5937–5941. doi: 10.1093/nar/20.22.5937

Mutagenesis analysis of the self-cleavage domain of hepatitis delta virus antigenomic RNA.

H N Wu 1, Z S Huang 1
PMCID: PMC334457  PMID: 1461726

Abstract

To determine the sequence requirements and structural features of the self-cleavage domain of hepatitis delta virus (HDV) antigenomic RNA, we constructed a series of mutants and measured the rate constant of the cleavage reaction for each. The self-cleavage activity of HDV RNA of antigenomic sense was found to reside in a region of less than 90 nucleotides in length. The catalytic domain contained a long complementary sequence which could be deleted to half of its original size. Moreover, this region could be replaced by other sequences as long as they could fold into a stem-and-loop structure. The catalytic domain also required a 6-basepair helix adjacent to the cleaving point for activity. The structural features of these two base-pairing regions are quite similar to those of the HDV genomic self-cleavage domain. The cleavage site as well as the the hinge region (the sequence between the two stems) requires specific sequences for activity.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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