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. 1992 Dec 11;20(23):6311–6315. doi: 10.1093/nar/20.23.6311

RNA-primed complementary-sense DNA synthesis of the geminivirus African cassava mosaic virus.

K Saunders 1, A Lucy 1, J Stanley 1
PMCID: PMC334521  PMID: 1475192

Abstract

The plant DNA virus African cassava mosaic virus (ACMV) is believed to replicate by a rolling circle mechanism. To investigate complementary-sense DNA (lagging strand) synthesis, we have analysed the heterogenous form of complementary-sense DNA (H3 DNA) from infected Nicotiana benthamiana by two-dimensional agarose gel electrophoresis and blot hybridisation. The presence of an RNA moeity is demonstrated by comparison of results for nucleic acids resolved on neutral/alkaline and neutral/formamide gels, suggesting that complementary-sense DNA synthesis on the virus-sense single-stranded DNA template is preceded by the synthesis of an RNA primer. Hybridisation with probes to specific parts of ACMV DNA A genome indicates that synthesis of the putative RNA primer initiates between nucleotides 2581-221, a region that includes intergenic sequences that have been implicated in geminivirus DNA replication and the control of gene expression.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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