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. 1992 Dec 25;20(24):6451–6454. doi: 10.1093/nar/20.24.6451

The TATA-binding protein participates in TFIIIB assembly on tRNA genes.

J Huet 1, A Sentenac 1
PMCID: PMC334556  PMID: 1480467

Abstract

The TATA-binding protein TBP has been recently recognized as a general class III transcription factor. Using the gel shift assay to monitor initiation complex assembly on a yeast tRNA gene, we show that TBP is required for the TFIIIC-dependent assembly of TFIIIB. TFIIIB depleted of TBP by a simple chromatographic step does not bind stably to the TFIIIC-tDNA complex. Addition of yeast or human recombinant TBP allows the formation of a TFIIIB-TBP-TFIIIC-tDNA complex. The presence of TBP in the complex was inferred from the effect of anti-TBP antibodies and from the different migration properties of TFIIIB-TBP-tDNA complexes formed with yeast or human TBP.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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