Abstract
The 5' region of the rRNA operon, rrnA, of M. capricolum was cloned. Sequence analysis revealed two tRNA genes, tRNA(leu) and tRNA(lys), upstream to the promoter of the rRNA operon. The in vivo transcription start sites of the rRNA operon and of the tRNA genes were mapped. The same promoters used by M. capricolum RNA polymerase are also recognized by E. coli RNA polymerase both in vivo and in vitro. We find that high levels of ppGpp in E. coli, resulting from amino acid starvation or from spoT mutation, activate rather than repress the transcription of the mycoplasma rrnA operon.
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- Amikam D., Glaser G., Razin S. Mycoplasmas (Mollicutes) have a low number of rRNA genes. J Bacteriol. 1984 Apr;158(1):376–378. doi: 10.1128/jb.158.1.376-378.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Devereux J., Haeberli P., Smithies O. A comprehensive set of sequence analysis programs for the VAX. Nucleic Acids Res. 1984 Jan 11;12(1 Pt 1):387–395. doi: 10.1093/nar/12.1part1.387. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Frischauf A. M., Lehrach H., Poustka A., Murray N. Lambda replacement vectors carrying polylinker sequences. J Mol Biol. 1983 Nov 15;170(4):827–842. doi: 10.1016/s0022-2836(83)80190-9. [DOI] [PubMed] [Google Scholar]
- Gadeau A. P., Mouches C., Bove J. M. Probable insensitivity of mollicutes to rifampin and characterization of spiroplasmal DNA-dependent RNA polymerase. J Bacteriol. 1986 Jun;166(3):824–828. doi: 10.1128/jb.166.3.824-828.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Glaser G., Amikam D., Razin S. Physical mapping of the ribosomal RNA genes of Mycoplasma capricolum. Nucleic Acids Res. 1984 Mar 12;12(5):2421–2426. doi: 10.1093/nar/12.5.2421. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Glaser G., Cashel M. In vitro transcripts from the rrn B ribosomal RNA cistron originate from two tandem promoters. Cell. 1979 Jan;16(1):111–121. doi: 10.1016/0092-8674(79)90192-2. [DOI] [PubMed] [Google Scholar]
- Glaser G., Razin A., Razin S. Stable RNA synthesis and its control in Mycoplasma capricolum. Nucleic Acids Res. 1981 Aug 11;9(15):3641–3646. doi: 10.1093/nar/9.15.3641. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Glaser G., Sarmientos P., Cashel M. Functional interrelationship between two tandem E. coli ribosomal RNA promoters. Nature. 1983 Mar 3;302(5903):74–76. doi: 10.1038/302074a0. [DOI] [PubMed] [Google Scholar]
- Henikoff S. Unidirectional digestion with exonuclease III creates targeted breakpoints for DNA sequencing. Gene. 1984 Jun;28(3):351–359. doi: 10.1016/0378-1119(84)90153-7. [DOI] [PubMed] [Google Scholar]
- Hyman H. C., Gafny R., Glaser G., Razin S. Transcription control elements of the Mycoplasma pneumoniae rRNA operon. Isr J Med Sci. 1987 Jun;23(6):585–590. [PubMed] [Google Scholar]
- Iwami M., Muto A., Yamao F., Osawa S. Nucleotide sequence of the rrnB 16S ribosomal RNA gene from Mycoplasma capricolum. Mol Gen Genet. 1984;196(2):317–322. doi: 10.1007/BF00328065. [DOI] [PubMed] [Google Scholar]
- Little R., Ryals J., Bremer H. rpoB mutation in Escherichia coli alters control of ribosome synthesis by guanosine tetraphosphate. J Bacteriol. 1983 May;154(2):787–792. doi: 10.1128/jb.154.2.787-792.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Loughney K., Lund E., Dahlberg J. E. Ribosomal RNA precursors of Bacillus subtilis. Nucleic Acids Res. 1983 Oct 11;11(19):6709–6721. doi: 10.1093/nar/11.19.6709. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Loughney K., Lund E., Dahlberg J. E. tRNA genes are found between 16S and 23S rRNA genes in Bacillus subtilis. Nucleic Acids Res. 1982 Mar 11;10(5):1607–1624. doi: 10.1093/nar/10.5.1607. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Lund E., Dahlberg J. E. Spacer transfer RNAs in ribosomal RNA transcripts of E. coli: processing of 30S ribosomal RNA in vitro. Cell. 1977 Jun;11(2):247–262. doi: 10.1016/0092-8674(77)90042-3. [DOI] [PubMed] [Google Scholar]
- Nene V., Glass R. E. Relaxed mutants of Escherichia coli RNA polymerase. FEBS Lett. 1983 Mar 21;153(2):307–310. doi: 10.1016/0014-5793(83)80630-9. [DOI] [PubMed] [Google Scholar]
- Ogasawara N., Moriya S., Yoshikawa H. Structure and organization of rRNA operons in the region of the replication origin of the Bacillus subtilis chromosome. Nucleic Acids Res. 1983 Sep 24;11(18):6301–6318. doi: 10.1093/nar/11.18.6301. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Panganiban A. T., Whiteley H. R. Purification and properties of a new bacillus subtilis RNA processing enzyme. Cleavage of phage SP82 mRNA and Bacillus subtilis precursor rRNA. J Biol Chem. 1983 Oct 25;258(20):12487–12493. [PubMed] [Google Scholar]
- Razin S. Molecular biology and genetics of mycoplasmas (Mollicutes). Microbiol Rev. 1985 Dec;49(4):419–455. doi: 10.1128/mr.49.4.419-455.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Riggs D. L., Mueller R. D., Kwan H. S., Artz S. W. Promoter domain mediates guanosine tetraphosphate activation of the histidine operon. Proc Natl Acad Sci U S A. 1986 Dec;83(24):9333–9337. doi: 10.1073/pnas.83.24.9333. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Salser W., Gesteland R. F., Bolle A. In vitro synthesis of bacteriophage lysozyme. Nature. 1967 Aug 5;215(5101):588–591. doi: 10.1038/215588a0. [DOI] [PubMed] [Google Scholar]
- Sanger F., Nicklen S., Coulson A. R. DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463–5467. doi: 10.1073/pnas.74.12.5463. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Sarmientos P., Cashel M. Carbon starvation and growth rate-dependent regulation of the Escherichia coli ribosomal RNA promoters: differential control of dual promoters. Proc Natl Acad Sci U S A. 1983 Nov;80(22):7010–7013. doi: 10.1073/pnas.80.22.7010. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Sarmientos P., Sylvester J. E., Contente S., Cashel M. Differential stringent control of the tandem E. coli ribosomal RNA promoters from the rrnA operon expressed in vivo in multicopy plasmids. Cell. 1983 Apr;32(4):1337–1346. doi: 10.1016/0092-8674(83)90314-8. [DOI] [PubMed] [Google Scholar]
- Sawada M., Muto A., Iwami M., Yamao F., Osawa S. Organization of ribosomal RNA genes in Mycoplasma capricolum. Mol Gen Genet. 1984;196(2):311–316. doi: 10.1007/BF00328064. [DOI] [PubMed] [Google Scholar]
- Taschke C., Herrmann R. Analysis of transcription and processing signals of the 16S-23S rRNA operon of Mycoplasma hyopneumoniae. Mol Gen Genet. 1986 Dec;205(3):434–441. doi: 10.1007/BF00338079. [DOI] [PubMed] [Google Scholar]