Skip to main content
PMC home page
Nucleic Acids Research logoLink to Nucleic Acids Research
. 1988 Jan 25;16(2):751–762. doi: 10.1093/nar/16.2.751

A laser Raman spectroscopic study of the interaction of calf-thymus DNA with Cu(II) and Pb(II) ions: metal ion binding and DNA conformational changes.

H A Tajmir-Riahi 1, M Langlais 1, R Savoie 1
PMCID: PMC334689  PMID: 3340554

Abstract

The interaction of calf-thymus DNA with Cu(II) and Pb(II) ions has been investigated in H2O and D2O solutions at physiological pH, using laser Raman spectroscopy. The results confirm the destabilizing effect of Cu2+ ions, which are shown to bind strongly to the guanine and cytidine bases, perturbing the A-T base pairs and disrupting the double-helical structure of DNA, whose conformation is markedly altered by these interactions. Earlier claims that Pb2+ ions destabilize DNA are not supported by the present study. These ions are found to interact only weakly with the nucleic bases, binding to the N7 position of the guanine bases and also interacting with the A-T pairs. Both types of ions are found to interact with the charged phosphate groups of DNA, although these sites are preferred over the nucleic bases by Pb2+ ions.

Full text

PDF
751

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bemski G., Rieber M., Wust M. ESR of Cu(2+) bound to polynucleotides and derivatives. FEBS Lett. 1971 Apr;14(2):117–120. doi: 10.1016/0014-5793(71)80115-1. [DOI] [PubMed] [Google Scholar]
  2. Benevides J. M., Thomas G. J., Jr Characterization of DNA structures by Raman spectroscopy: high-salt and low-salt forms of double helical poly(dG-dC) in H2O and D2O solutions and application to B, Z and A-DNA. Nucleic Acids Res. 1983 Aug 25;11(16):5747–5761. doi: 10.1093/nar/11.16.5747. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Benevides J. M., Wang A. H., van der Marel G. A., van Boom J. H., Rich A., Thomas G. J., Jr The Raman spectra of left-handed DNA oligomers incorporating adenine-thymine base pairs+. Nucleic Acids Res. 1984 Jul 25;12(14):5913–5925. doi: 10.1093/nar/12.14.5913. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. EICHHORN G. L., CLARK P. INTERACTIONS OF METAL IONS WITH POLYNUCLEOTIDES AND RELATED COMPOUNDS. V. THE UNWINDING AND REWINDING OF DNA STRANDS UNDER THE INFLUENCE OF COPPER (II) IONS. Proc Natl Acad Sci U S A. 1965 Mar;53:586–593. doi: 10.1073/pnas.53.3.586. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. EICHHORN G. L. Metal ions as stabilizers or destabilizers of the deoxyriboucleic acid structure. Nature. 1962 May 5;194:474–475. doi: 10.1038/194474a0. [DOI] [PubMed] [Google Scholar]
  6. Eichhorn G. L., Shin Y. A. Interaction of metal ions with polynucleotides and related compounds. XII. The relative effect of various metal ions on DNA helicity. J Am Chem Soc. 1968 Dec 18;90(26):7323–7328. doi: 10.1021/ja01028a024. [DOI] [PubMed] [Google Scholar]
  7. Erfurth S. C., Kiser E. J., Peticolas W. L. Determination of the backbone structure of nucleic acids and nucleic acid oligomers by laser Raman scattering. Proc Natl Acad Sci U S A. 1972 Apr;69(4):938–941. doi: 10.1073/pnas.69.4.938. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Erfurth S. C., Peticolas W. L. Melting and premelting phenomenon in DNA by laser Raman scattering. Biopolymers. 1975 Feb;14(2):247–264. doi: 10.1002/bip.1975.360140202. [DOI] [PubMed] [Google Scholar]
  9. Goodwin D. C., Brahms J. Form of DNA and the nature of interactions with proteins in chromatin. Nucleic Acids Res. 1978 Mar;5(3):835–850. doi: 10.1093/nar/5.3.835. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Lanir A., Yu N. T. A Raman spectroscopic study of the interaction of divalent metal ions with adenine moiety of adenosine 5'-triphosphate. J Biol Chem. 1979 Jul 10;254(13):5882–5887. [PubMed] [Google Scholar]
  11. Mansy S., Peticolas W. L. Detection of the sites of alkylation in DNA and polynucleotides by laser Raman spectroscopy. Biochemistry. 1976 Jun 15;15(12):2650–2655. doi: 10.1021/bi00657a026. [DOI] [PubMed] [Google Scholar]
  12. Mansy S., Tobias R. S. Heavy metal-nucleotide interactions. II. Binding of methylmercury(II) to purine nucleosides and nucleotides studied by Raman difference spectroscopy. J Am Chem Soc. 1974 Oct 30;96(22):6874–6885. doi: 10.1021/ja00829a011. [DOI] [PubMed] [Google Scholar]
  13. Maskos K. Interaction of metal ions with nucleic acids. Interaction of copper(II) with inosine and its derivatives. Acta Biochim Pol. 1978;25(2):113–127. [PubMed] [Google Scholar]
  14. O'Connor T., Bina M., McMillin D. R., Moller Haley M. R., Tobias R. S. Heavy metal-nucleotide interactions. 15. Reactions of calf thymus DNA with the electrophiles methylmercury(II) nitrate, cis-dichlorodiammineplatinum(II), and trans-dichlorodiammineplatinum(II) studied using Raman difference spectroscopy. Evidence for the formation of c-DNA upon metalation. Biophys Chem. 1982 Jun;15(3):223–234. [PubMed] [Google Scholar]
  15. Prescott B., Steinmetz W., Thomas G. J., Jr Characterization of DNA structures by laser Raman spectroscopy. Biopolymers. 1984 Feb;23(2):235–256. doi: 10.1002/bip.360230206. [DOI] [PubMed] [Google Scholar]
  16. Richard H., Schreiber J. P., Daune M. Interactions of metallic ions with DNA. V. DNA renaturation mechanism in the presence of Cu 2+ . Biopolymers. 1973;12(1):1–10. doi: 10.1002/bip.1973.360120102. [DOI] [PubMed] [Google Scholar]
  17. Zimmer C., Luck G., Fritzsche H., Triebel H. DNA-copper (II) complex and the DNA conformation. Biopolymers. 1971;10(3):441–463. doi: 10.1002/bip.360100303. [DOI] [PubMed] [Google Scholar]

Articles from Nucleic Acids Research are provided here courtesy of Oxford University Press

RESOURCES