Figure 2.

Refolding of EAV proteins during dialysis of urea-extracts of inclusion bodies. Upper panel: Dialysis of individual EAV-proteins. Lower panel: Combined dialysis of the indicated combinations of EAV-proteins. Inclusion bodies were resuspended in denaturating buffer. After centrifugation the resulting supernatants were dialyzed against buffer with decreasing urea concentration and finally against physiological buffer. The dialysate was then centrifuged and aliquots of the supernatant (S) and of the pellet (P) were analyzed by SDS-PAGE under reducing conditions and Coomassie-staining. M: molecular mass marker as indicated, Ly: Lysozyme. Due to the lack of antibodies we were not able to confirm the identiy of the bands by Western blot. Nevertheless, since the recombinant proteins are the major proteins present in bacterial lysates (see Figure 1), we are sure that the identification of bands is correct.