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. 2012 Apr 16;4(4):581–612. doi: 10.3390/v4040581

Figure 4.

Figure 4

Increased expression of the Kelch domain containing 7B (KLHDC7B, Chr 22, + strand) gene during acute HCV infection. (A) The RNA-seq analysis identified an 18.9-fold increase KLHDC7B mRNA with an FDR value of 141.9. The gene structure, orientation and chromosomal location are shown below the sequencing reads. This example shows one benefit of analyzing 5' capped by RNA sequencing and provides evidence that an isoform of KLHDC7B mRNA with a longer 5' untranslated region (UTR) is expressed in Huh 7.5 cells after infection with HCV. There was no annotated gene or EST on the minus strand in this genomic region that provided an alternative explanation for the sequence reads observed upstream of the KLHDC7B 5' end. (B) qPCR analysis verified a major increase in expression of KLHDC7B and showed a 9-fold increase mRNA with the PCR primers used.