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. 2001 May 22;98(11):6435–6440. doi: 10.1073/pnas.111051398

Figure 3.

Figure 3

Competitive reverse transcription–PCR of transgene-derived p27Kip1 mRNA in the forebrains of dox-exposed E12 and E13 DT embryos. (a) Lane 1, competitor cRNA alone; lane 2, E12 forebrain mRNA (sample) alone; lanes 3–7, sample mRNA containing 2-fold serial increments of competitor cRNA. (b) The logarithm of the ratios of sample to competitor (vertical axis) was plotted against the logarithm of the amounts of competitor (horizontal axis). A ratio of 0 represents the concentration of transgene-specific p27Kip1 mRNA in the sample. (c) Dose-dependent increase in transgene-specific mRNA after 24 h of dox exposure, beginning on E12. (d) Regulatablity of transgene-specific transcript induction after a 25 μg/g bw−1 dose of dox on E12. Each bar represents mean values from two independent experiments.