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. 1989 Nov 11;17(21):8669–8682. doi: 10.1093/nar/17.21.8669

In vivo transcription of a cloned prosimian primate SINE sequence.

V K Slagel 1, P L Deininger 1
PMCID: PMC335035  PMID: 2479909

Abstract

SINE transcription was studied by introducing a galago Monomer family member (gal39), into the mouse Ltk- cell line. Cells transiently transfected with gal39 produce gal39-specific RNA polymerase III-directed transcripts. In permanent cell lines gal39 expression was largely shut off. Genomic environment, copy number and tandem repetition of integrated SINE sequences influenced whether or not RNA polymerase III-directed gal39 transcripts were detectable. These transcripts differ in length from the observed endogenous transcript in cultured galago cells which hybridizes to this repetitive DNA family.

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