Figure S2.

PcG Core Components and the Associated Histone Marks Are Enriched at the FSHD Locus, Related to Figure 1

ChIP-qPCR experiments with antibodies specific for EZH2 (A) and its associated histone mark, H3K27me3 (B), in primary muscle cells from 4 healthy donors and 4 FSHD patients. ChIP was analyzed by qPCR with primers specific for the promoters of 4q35 genes ANT1, FRG1 and FRG2. NDE is shown as comparison of the enrichment in the FSHD locus.

Results are expressed as percentage of input (EZH2), or percentage of total H3 (H3K27me3). The mean of the signals obtained from 4 healthy samples or 4 FSHD patients is shown. The error bars represent SEM.

(C) Scheme of the FSHD locus. The location of the primers used for ChIP-qPCR is shown.

(D-G) ChIP-qPCR experiments with antibodies specific for the core components of PRC1 and H2Aub1 (D), PRC2 and H3K27me3 (E), and Jarid2 and c-Krox/Th-POK (F) and macroH2A (G) in human chr4/CHO. Hoxd11 is shown as positive control. ChIP material was analyzed by qPCR with primers for different regions of the D4Z4 repeat and for NDE (see scheme C). Results are expressed as percentage of input (PcG proteins and macroH2A), percentage of the ChIP signal obtained with anti-H3 antibodies (H3K27me3) or percentage of the ChIP signal obtained with anti-H2A antibodies and normalized for IgM (H2Aub1).

The error bars represent SEM.