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. 1989 Nov 25;17(22):9395–9405. doi: 10.1093/nar/17.22.9395

Structure and functional expression of a cloned Xenopus thyroid hormone receptor.

A R Brooks 1, G Sweeney 1, R W Old 1
PMCID: PMC335140  PMID: 2587261

Abstract

A clone corresponding to a thyroid hormone receptor was isolated from a Xenopus laevis cDNA library prepared from folliculated oocytes. The cDNA encodes a protein of 418 amino acid residues with a domain structure, including a putative DNA binding region with two zinc fingers, similar to other members of the v-erbA-related superfamily of receptors. The encoded protein resembles the TR alpha 1-type receptor of the rat. When expressed in COS cells the protein product binds triiodothyronine with a Kd of 0.12 nM. The receptor mediates thyroid-hormone-inducible expression of a reporter gene which includes a thyroid hormone response element in its upstream region.

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Selected References

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