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. 1989 Nov 25;17(22):9407–9415. doi: 10.1093/nar/17.22.9407

A negative regulatory element in the promoter of the human alpha 1-antitrypsin gene.

V De Simone 1, R Cortese 1
PMCID: PMC335141  PMID: 2555792

Abstract

A "minimal tissue-specific element" of the human alpha 1-antitrypsin (alpha 1-AT) gene promoter, located in the -137/-37 region, is able to drive hepatoma-specific transcription from the heterologous SV40 promoter. Here we show that, in HeLa cells, this element is inactivated by a negative regulatory mechanism. This negative control can be overcome by deletion or mutation of two specific DNA domains.

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Selected References

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