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. 2012 May 14;61(6):1509–1518. doi: 10.2337/db11-0134

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© 2012 by the American Diabetes Association.

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FIG. 4. — A: Cell lysate analysis by Luminex assay of CD4⁺ T-cells retrieved from the in vitro Treg-generation assay (anti-CD3/CD28 stimulation and TGF-β) at 20 min showed an increase in the phosphorylation of the nuclear transcription factor CREB under PI3Kγ pathway inhibition compared with untreated control (data represent one of three separate experiments). B: Representative example of FACS staining showing the effect of the CREB inhibition on Treg generation in vitrousing a CD3/CD28 stimulation assay with TGF-β with or without PI3Kγ-i (data represent one out of three separate experiments). The percentages of Tregs from these different conditions are shown in the bar graph in C (*P < 0.05; n = 3–5 mice; data are representative of three separate experiments). Results are presented as the mean ± SEM. (A high-quality color representation of this figure is available in the online issue.)