Skip to main content
Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1967 Sep;58(3):1189–1196. doi: 10.1073/pnas.58.3.1189

Synthesis of gamma G antibody and immunoglobulin on polyribosomes in a cell-free system.

J P Gusdon Jr, A B Stavitsky, S A Armentrout
PMCID: PMC335767  PMID: 4167890

Full text

PDF
1189

Images in this article

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. ALLEN E. H., SCHWEET R. S. Synthesis of hemoglobin in a cell-free system. I. Properties of the complete system. J Biol Chem. 1962 Mar;237:760–767. [PubMed] [Google Scholar]
  2. BAUER D. C., MATHIES M. J., STAVITSKY A. B. Sequences of synthesis of gamma-1 macroglobulin and gamma-2 globulin antibodies during primary and secondary responses to proteins, salmonella antigens, and phage. J Exp Med. 1963 Jun 1;117:889–907. doi: 10.1084/jem.117.6.889. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. BAUER D. C. THE MOLECULAR PROPERTIES OF ANTIBODIES SYNTHESIZED IN RESPONSE TO SIMPLE HAPTENES. J Immunol. 1963 Sep;91:323–330. [PubMed] [Google Scholar]
  4. Britten R. J., Roberts R. B. High-Resolution Density Gradient Sedimentation Analysis. Science. 1960 Jan 1;131(3392):32–33. doi: 10.1126/science.131.3392.32. [DOI] [PubMed] [Google Scholar]
  5. DANIEL T. M., STAVITSKY A. B. PASSIVE HEMAGGLUTINATION IN STUDY OF ANTIGENS AND ANTIBODIES. Methods Med Res. 1964;10:152–157. [PubMed] [Google Scholar]
  6. DURE L., WATERS L. LONG-LIVED MESSENGER RNA: EVIDENCE FROM COTTON SEED GERMINATION. Science. 1965 Jan 22;147(3656):410–412. doi: 10.1126/science.147.3656.410. [DOI] [PubMed] [Google Scholar]
  7. Freeman M. J., Stavitsky A. B. Radioimmunoelectrophoretic study of rabbit anti-protein antibodies during the primary response. J Immunol. 1965 Dec;95(6):981–990. [PubMed] [Google Scholar]
  8. GROSS P. R., COUSINEAU G. H. Effects of actinomycin D on macromolecule synthesis and early development in sea urchin eggs. Biochem Biophys Res Commun. 1963 Feb 18;10:321–326. doi: 10.1016/0006-291x(63)90532-1. [DOI] [PubMed] [Google Scholar]
  9. HELMREICH E., KERN M., EISEN H. N. The secretion of antibody by isolated lymph node cells. J Biol Chem. 1961 Feb;236:464–473. [PubMed] [Google Scholar]
  10. Kern M., Helmreich E., Eisen H. N. A DEMONSTRATION OF ANTIBODY ACTIVITY ON MICROSOMES. Proc Natl Acad Sci U S A. 1959 Jun;45(6):862–867. doi: 10.1073/pnas.45.6.862. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  12. Lazda V., Starr J. L. The stability of messenger ribonucleic acid in antibody synthesis. J Immunol. 1965 Aug;95(2):254–261. [PubMed] [Google Scholar]
  13. Mach B., Vassalli P. Biosynthesis of RNA in antibody-producing tissues. Proc Natl Acad Sci U S A. 1965 Sep;54(3):975–982. doi: 10.1073/pnas.54.3.975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. PALMER J. L., NISONOFF A., VANHOLDE K. E. DISSOCIATION OF RABBIT GAMMA GLOBULIN INTO SUBUNITS BY REDUCTION AND ACIDIFICATION. Proc Natl Acad Sci U S A. 1963 Aug;50:314–321. doi: 10.1073/pnas.50.2.314. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Plagemann P. G., Swim H. E. Replication of mengovirus. I. Effect on synthesis of macromolecules by host cell. J Bacteriol. 1966 Jun;91(6):2317–2326. doi: 10.1128/jb.91.6.2317-2326.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. SCHARFF M. D., UHR J. W. FUNCTIONAL RIBOSOMAL UNIT OF GAMMA-GLOBULIN SYNTHESIS. Science. 1965 Apr 30;148(3670):646–648. doi: 10.1126/science.148.3670.646. [DOI] [PubMed] [Google Scholar]
  17. SCHEIDEGGER J. J. Une micro-méthode de l'immuno-electrophorèse. Int Arch Allergy Appl Immunol. 1955;7(2):103–110. [PubMed] [Google Scholar]
  18. Shapiro A. L., Scharff M. D., Maizel J. V., Jr, Uhr J. W. Polyribosomal synthesis and assembly of the H and L chains of gamma globulin. Proc Natl Acad Sci U S A. 1966 Jul;56(1):216–221. doi: 10.1073/pnas.56.1.216. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Stavitsky A. B., Gusdon J. P., Jr Symposium on in vitro studies of the immune response. IV. Role of nucleic acids in the anamnestic antibody response. Bacteriol Rev. 1966 Jun;30(2):418–426. doi: 10.1128/br.30.2.418-426.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Stenzel K. H., Rubin A. L. Biosynthesis of gamma globulin: studies in a cell-free system. Science. 1966 Jul 29;153(3735):537–539. doi: 10.1126/science.153.3735.537. [DOI] [PubMed] [Google Scholar]
  21. WEISBERGER A. S., WOLFE S., ARMENTROUT S. INHIBITION OF PROTEIN SYNTHESIS IN MAMMALIAN CELL-FREE SYSTEMS BY CHLORAMPHENICOL. J Exp Med. 1964 Aug 1;120:161–181. doi: 10.1084/jem.120.2.161. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. WUST C. J., GALL C. L., NOVELLI D. G. ACTINOMYCIN D: EFFECT ON THE IMMUNE RESPONSE. Science. 1964 Mar 6;143(3610):1041–1043. doi: 10.1126/science.143.3610.1041. [DOI] [PubMed] [Google Scholar]
  23. Weisberger A. S., Armentrout S. A. Directed protein synthesis by messenger ribonucleoprotein and ribosomes from different mammalian species. Proc Natl Acad Sci U S A. 1966 Nov;56(5):1612–1619. doi: 10.1073/pnas.56.5.1612. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Williamson A. R., Askonas B. A. Biosynthesis of immunoglobulins: the separate classes of polyribosomes synthesizing heavy and light chains. J Mol Biol. 1967 Jan 28;23(2):201–216. doi: 10.1016/s0022-2836(67)80027-5. [DOI] [PubMed] [Google Scholar]
  25. Zucker W. V., Schulman H. M. The synthesis of globin dimers by a reticulocyte cell-free system. Biochim Biophys Acta. 1967 Apr 18;138(2):400–410. doi: 10.1016/0005-2787(67)90499-6. [DOI] [PubMed] [Google Scholar]

Articles from Proceedings of the National Academy of Sciences of the United States of America are provided here courtesy of National Academy of Sciences

RESOURCES