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. 2012 Jun 15;139(12):2118–2129. doi: 10.1242/dev.076067

Fig. 3.

Fig. 3.

Tcf3–β-catenin interaction is required for post-axial digit formation. (A-B′) Gross morphology of autopod limb segment in WT (A,B) and KI (A′,B′) mouse limbs at E16.5 (A,A′) and P1 (B,B′). Limbs were stained with Alizarin Red (B,B′) and numbers correspond to anterior (#1) to posterior (#5) digits. (C,C′) In situ hybridization of limb buds from E10.5 (C) and E11.5 (C′) WT embryos using a cRNA probe for Tcf3. (D-E′) Whole-mount in situ hybridization using a cRNA probe for Tbx2 mRNA on E11.5 (D,D′) and E12.5 (E,E′) embryos. The posterior edge of Tbx2 expression (arrows) in WT limb buds (D,E) was absent in KI limb buds (D′,E′). (F,F′) Tcf3–β-catenin activity was detected with the BAT-Gal transgene in WT (F) and KI (F′) limb buds at E10.0 [31-somite stage (ss)]. Arrows point to the high BAT-Gal activity in the posterior mesenchyme region of WT limb buds (F), which is absent from KI limb buds (F′).