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. 1970 Aug;66(4):1183–1189. doi: 10.1073/pnas.66.4.1183

IMMUNOGLOBULIN SYNTHESIS AND SECRETION, V. INCORPORATION OF LEUCINE AND GLUCOSAMINE INTO IMMUNOGLOBULIN ON FREE AND BOUND POLYRIBOSOMES*

Charles J Sherr 1,†, Jonathan W Uhr 1
PMCID: PMC335804  PMID: 5273448

Abstract

Mouse myeloma cells pulse-labeled in vitro with 3H-leucine or 3H-glucosamine were fractionated on sucrose gradients, and membrane-bound and free polyribosomes were separated. Nascent polypeptides released from polyribosomes were precipitated with antiserum specific for mouse immunoglobulin. The results indicate that immunoglobulin is synthesized preferentially by bound polyribosomes.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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