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. 1970 Aug;66(4):1213–1219. doi: 10.1073/pnas.66.4.1213

PROINSULIN: CRYSTALLIZATION AND PRELIMINARY X-RAY DIFFRACTION STUDIES*

W Wardle Fullerton 1, Reginald Potter 1,†, Barbara W Low 1,‡
PMCID: PMC335808  PMID: 5273450

Abstract

Bovine proinsulin has been crystallized under a variety of conditions at both neutral and acid pH. Microtechniques were employed with sample weights of about 200 μg and volumes of 5-20 μl. The crystalline preparations all differ from each other morphologically.

X-ray photographs of one form, tetragonal bipyramids grown at pH 3 with added ammonium sulphate solution, established the space group P41212 (or its enantiomorph P43212). The cell dimensions are a = 50.8 ± 0.2 Å, c = 148.0 ± 0.4 Å. The asymmetric unit in this form is a dimer of proinsulin which is also the dominant species in solution at this pH.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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