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. 1970 Aug;66(4):1220–1227. doi: 10.1073/pnas.66.4.1220

MAPPING OF A HUMAN GENETIC REGULATOR ELEMENT BY SOMATIC CELL GENETIC ANALYSIS*

Robert J Klebe 1,†, Tchaw-ren Chen 1, Frank H Ruddle 1
PMCID: PMC335809  PMID: 4920091

Abstract

The ES-2 esterase is a kidney-associated esterase and is expressed in a cloned mouse renal adenocarcinoma cell line. Somatic cell hybridization of the mouse renal adenocarcinoma with either mouse or human fibroblasts leads to the marked reduction of ES-2 esterase titer. The extinction of ES-2 esterase activity in mouse×human hybrids is reversible. Extinction correlates with the presence of the human C10 chromosome in the somatic cell hybrids; whereas reexpression of the ES-2 esterase is observed in hybrids which have lost C10. Thus, the proposed regulator element involved is probably structurally linked to C10; however, linkage to a member of the D or G groups cannot be completely excluded.

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