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. 1970 Aug;66(4):1228–1235. doi: 10.1073/pnas.66.4.1228

CHANGES IN CHROMOSOMES OF BONE MARROW AFTER INTRAVENOUS INJECTIONS OF 7,12-DIMETHYLBENZ(A)ANTHRACENE AND RELATED COMPOUNDS*

E Douglas Rees 1,2,†, S K Majumdar 1,2, Amy Shuck 1,2
PMCID: PMC335810  PMID: 5273451

Abstract

Multiple intravenous injections of an emulsion containing 7,12-dimethylbenz(a)anthracene (DMBA) or 7,8,12-trimethylbenz(a)anthracene (TMBA) induce a high incidence of leukemia in rats. Twenty-four hours after a single injection, about half of the metaphase cells in the marrow have chromosomes with breaks. Although breaks were inflicted on chromosomes of various sizes and morphology, these aberrations were nonrandom in that members of the nos. 1 and 2 chromosome pairs were involved to an extent greater than expected on the basis of their size and number. Distinctive karyotypic abnormalities involving the no. 2 chromosome were observed in half of the leukemic rats, whereas these abnormalities were not observed in nonleukemic, DMBA-treated rats. Benzo(a)pyrene and benzo(e)pyrene, polycyclic aromatic hydrocarbons which did not induce leukemia, produced fewer breaks of the no. 2 (and other) chromosomes than did DMBA or TMBA.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. DUNN G. D., MOLONEY W. C. CHROMOSOME STUDIES IN NORMAL AND LEUKEMIC RATS. Blood. 1964 May;23:564–571. [PubMed] [Google Scholar]
  2. HUNGERFORD D. A., NOWELL P. C. SEX CHROMOSOME POLYMORPHISM AND THE NORMAL KARYOTYPE IN THREE STRAINS OF THE LABORATORY RAT. J Morphol. 1963 Sep;113:275–285. doi: 10.1002/jmor.1051130213. [DOI] [PubMed] [Google Scholar]
  3. Huggins C. B., Grand L. Neoplasms evoked in male Sprague-Dawley rat by pulse doses of 7,12-dimethylbenz(a)anthracene. Cancer Res. 1966 Nov;26(11):2255–2258. [PubMed] [Google Scholar]
  4. Huggins C. B., Sugiyama T. Induction of leukemia in rat by pulse doses of 7,12-dimethylbenz(a)anthracene. Proc Natl Acad Sci U S A. 1966 Jan;55(1):74–81. doi: 10.1073/pnas.55.1.74. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Huggins C., Grand L., Oka H. Hundred day leukemia: preferential induction in rat by pulse-doses of 7,8,12-trimethylbenz(a)anthracene. J Exp Med. 1970 Feb;131(2):321–330. doi: 10.1084/jem.131.2.321. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Kato R., Bruze M., Tegner Y. Chromosome breakage induced in vivo by a carcinogenic hydrocarbon in bone marrow cells of the Chinese hamster. Hereditas. 1969;61(1):1–8. doi: 10.1111/j.1601-5223.1969.tb01833.x. [DOI] [PubMed] [Google Scholar]
  7. Kato R. Chromosome breakage induced by a carcinogenic hydrocarbon in Chinese hamster cells and human leukocytes in vitro. Hereditas. 1968;59(1):120–141. doi: 10.1111/j.1601-5223.1968.tb02167.x. [DOI] [PubMed] [Google Scholar]
  8. Kuri Y., Sugiyama T., Nishizuka Y. Chromosome aberrations induced in rat bone marrow cells by 7,12-dimethylbenz[a]-anthracene. J Natl Cancer Inst. 1969 Sep;43(3):635–641. [PubMed] [Google Scholar]
  9. Kurita Y., Sugiyama T., Nishizuka Y. Cytogenetic analysis of cell populations in rat leukemia induced by pulse doses of 7,12-dimethylbenz(a)anthracene. Gan. 1969 Oct;60(5):529–535. [PubMed] [Google Scholar]
  10. Kurita Y., Sugiyama T., Nishizuka Y. Cytogenetic studies on rat leukemia induced by pulse doses of 7,12-dimethylbenz(alpha)anthracene. Cancer Res. 1968 Sep;28(9):1738–1752. [PubMed] [Google Scholar]
  11. MOLONEY W. C., BOSCHETTI A. E., DOWD G. OBSERVATIONS ON LEUKEMIA IN WISTAR AND WISTAR FURTH RATS. Blood. 1965 Sep;26:341–353. [PubMed] [Google Scholar]
  12. MOORHEAD P. S., NOWELL P. C., MELLMAN W. J., BATTIPS D. M., HUNGERFORD D. A. Chromosome preparations of leukocytes cultured from human peripheral blood. Exp Cell Res. 1960 Sep;20:613–616. doi: 10.1016/0014-4827(60)90138-5. [DOI] [PubMed] [Google Scholar]
  13. NOWELL P. C., FERRY S., HUNGER FORD D. A. Chromosomes of primary granulocytic leukemia (chloroleukemia) in the rat. J Natl Cancer Inst. 1963 Apr;30:687–703. [PubMed] [Google Scholar]
  14. POGOSIANZ H. E., EGOLINA N. A., FICHIDZIAN B. S., PLATONOVA G. M., VOITOVIZKI V. K. CHROMOSOME CHARACTERISTICS OF SOME NEW TRANSPLANTABLE TUMORS AND LEUKEMIAS. Acta Unio Int Contra Cancrum. 1964;20:1347–1351. [PubMed] [Google Scholar]
  15. Rees E. D., Shick A. E., Christian J. C., Pugh J. R. Karyotypes of rats from strains of different susceptibility to mammary cancer induction. Cancer Res. 1968 May;28(5):823–830. [PubMed] [Google Scholar]
  16. Rubibini J. R., Yakaitis A. A marker chromosome in transplantable rat leukemia, R3149. Proc Soc Exp Biol Med. 1967 May;125(1):1–4. doi: 10.3181/00379727-125-31998. [DOI] [PubMed] [Google Scholar]
  17. Schurr P. E. Composition and preparation of experimental intravenous fat emulsions. Cancer Res. 1969 Jan;29(1):258–260. [PubMed] [Google Scholar]
  18. Sugiyama T., Brillantes F. P. Cytogenetic studies of leukemia induced by 6,8,12-and 7,8,12-trimethylbenz(a)anthracene. J Exp Med. 1970 Feb;131(2):331–341. doi: 10.1084/jem.131.2.331. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Sugiyama T., Kurita Y., Nishizuka Y. Biologic studies on 7,12-dimethylbenz(alpha) anthracene-induced rat leukemia with special reference to the specific chromosomal abnormalities. Cancer Res. 1969 May;29(5):1117–1124. [PubMed] [Google Scholar]
  20. Sugiyama T., Kurita Y., Nishizuka Y. Chromosome abnormality in rat leukemia induced by 7,12-dimethylbenz[a]anthracene. Science. 1967 Nov;158(3804):1058–1059. doi: 10.1126/science.158.3804.1058. [DOI] [PubMed] [Google Scholar]
  21. Yakaitis A. A., Rubini J. R. Marker chromosomes in IRC 741 rat leukemia. Cancer Res. 1967 Dec;27(12):2377–2380. [PubMed] [Google Scholar]

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