Figure 6.

Long-term (20 weeks) but not intermediate (6-13 weeks) human platelet- and neutrophil-producing activities in different samples of CB correlate with one another. (A) NSG mice were each transplanted with 5000-10 000 Lin⁻ALDH⁺ CB cells or the cells from the matching fraction of Lin⁻ALDH⁻ cells and the number of circulating human platelets (left panel) or human neutrophils in the BM (right panel) was determined up to 20 weeks later. The number above each bar indicates the proportion of total platelet- or neutrophil-producing cell activity in that cell fraction. Values shown are the mean ± SEM of data from 3 experiments. (B) Lin⁻ALDH⁺ cells from 7 different CB preparations were transplanted into NSG mice and the outputs of human platelets (in PB) and neutrophils (in BM aspirates) in each mouse were determined at various time points later. Pearson correlation analyses showed that these values were significantly correlated only for the week 20 posttransplantation end point.