Table 1.
Quantum yield and extinction coefficient for commonly-use fluorescent probes
| Fluorochrome | Quantum Yield | Molar Extinction Coefficienta | Excitation/ Absorbance |
|---|---|---|---|
| Brilliant Violet | 0.69 | 2,500,000 | Violet (405 nm) |
| R-PE | 0.82 | 1,960,000 | Blue (496 nm) |
| APC | 0.68 | 700,000 | Red (650 nm) |
| Quantum Dot 655 | ≈0.3b | 5,700,000 | Violet (405 nm) |
| Quantum Dot 585 | ≈0.7b | 2,200,000 | Violet (405 nm) |
| AlexaFluor 660 | 0.37 | 132,000 | Red (663 nm) |
| Fluorescein | 0.5 | 86,000 | Blue (488 nm) |
| AlexaFluor 488 | 0.92 | 71,000 | Blue (495 nm) |
| Pacific Blue | 0.78 | 46,000 | Violet (405 nm) |
a
Measured at the indicated excitation/absorbance wavelength, cm-1M-1
b
Quantum yields generally increase for larger Quantum Dots (11)