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. 1976 Jan;73(1):218–222. doi: 10.1073/pnas.73.1.218

Isoimmunization against human chorionic gonadotropin with conjugates of processed beta-subunit of the hormone and tetanus toxoid.

G P Talwar, N C Sharma, S K Dubey, M Salahuddin, C Das, S Ramakrishnan, S Kumar, V Hingorani
PMCID: PMC335872  PMID: 813223

Abstract

The immunogenicity of the conjugate prepared from "processed" beta-subunit of human chorionic gonadotropin (choriogonadotropin, HCG) and tetanus toxoid has been studied in animals and a human subject. The conjugate elicited the formation of high-affinity (Ka = 10(9)-10(11) M-1) anti-HCG and anti-tetanus antibodies. On primary immunization, the antibody, response lasted for several months. Repeat injection of the conjugate in the declining phase of antibody titers produced a booster response without a lag period. The antibodies reacted with the beta-subunit of HCG and the complete HCG molecule but were devois of significant crossreactivity with human growth hormone, placental lactogen, follicle-stimulating hormone, thyroid-stimulating hormone, and luteinizing hormone at tonic and surge levels. The antibodies were competent for neutralizing the biological activity of HCG in the mouse uterine weight gain assay, the ventral prostate weight gain assay, and the radioligand assay for binding of 125I-labeled HCG to receptors on corpus luteum. HCG (5000 international units) administered to an immunized subject was completely bound by circulating antibodies. Administration of HCG (in contrast to conjugate) was without booster effect on anti-HCG titers.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Beall G. N., Chopra I. J., Solomon D. H., Pierce J. G., Cornell J. S. Neutralizing and non-neutralizing antibodies to bovine thyroid-stimulating hormone and its subunits. J Clin Invest. 1973 Dec;52(12):2979–2985. doi: 10.1172/JCI107495. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Braunstein G. D., Bridson W. E., Glass A., Hull E. W., McIntire K. R. In vivo and in vitro production of human chorionic gonadotropin and alpha-feteoprotein by a virilizing hepatoblastoma. J Clin Endocrinol Metab. 1972 Dec;35(6):857–862. doi: 10.1210/jcem-35-6-857. [DOI] [PubMed] [Google Scholar]
  3. Braunstein G. D., Vaitukaitis J. L., Carbone P. P., Ross G. T. Ectopic production of human chorionic gonadotrophin by neoplasms. Ann Intern Med. 1973 Jan;78(1):39–45. doi: 10.7326/0003-4819-78-1-39. [DOI] [PubMed] [Google Scholar]
  4. Canfield R. E., Morgan F. J., Kammerman S., Bell J. J., Agosto G. M. Studies of human chorionic gonadotropin. Recent Prog Horm Res. 1971;27:121–164. doi: 10.1016/b978-0-12-571127-2.50028-6. [DOI] [PubMed] [Google Scholar]
  5. Crawford J. W. Follow-up of hydatidiform mole by radioimmunoassay of human chorionic gonadotrophin. Br Med J. 1972 Dec 23;4(5842):715–719. doi: 10.1136/bmj.4.5842.715. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Louvet J. P., Ross G. T., Birken S., Canfield R. E. Absence of neutralizing effect of antisera to the unique structural region of human chorionic gonadotropin. J Clin Endocrinol Metab. 1974 Dec;39(6):1155–1158. doi: 10.1210/jcem-39-6-1155. [DOI] [PubMed] [Google Scholar]
  7. Saxena B. B., Hasan S. H., Haour F., Schmidt-Gollwitzer M. Radioreceptor assay of human chorionic gonadotropin: detection of early pregnancy. Science. 1974 May 17;184(4138):793–795. doi: 10.1126/science.184.4138.793. [DOI] [PubMed] [Google Scholar]
  8. Skelley D. S., Brown L. P., Besch P. K. Radioimmunoassay. Clin Chem. 1973 Feb;19(2):146–186. [PubMed] [Google Scholar]
  9. Stevens V. C., Crystle C. D. Effects of immunization with hapten-coupled HCG on the human menstrual cycle. Obstet Gynecol. 1973 Oct;42(4):485–495. doi: 10.1097/00006250-197310000-00001. [DOI] [PubMed] [Google Scholar]
  10. Stevens V. C. Immunization of female baboons with hapten-coupled gonadotropins. Obstet Gynecol. 1973 Oct;42(4):496–506. doi: 10.1097/00006250-197310000-00002. [DOI] [PubMed] [Google Scholar]
  11. Vaitukaitis J. L., Braunstein G. D., Ross G. T. A radioimmunoassay which specifically measures human chorionic gonadotropin in the presence of human luteinizing hormone. Am J Obstet Gynecol. 1972 Jul 15;113(6):751–758. doi: 10.1016/0002-9378(72)90553-4. [DOI] [PubMed] [Google Scholar]

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