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. 1976 Feb;73(2):423–427. doi: 10.1073/pnas.73.2.423

A method for determination of the in situ distribution of chromosomal proteins.

L M Silver, S C Elgin
PMCID: PMC335921  PMID: 813226

Abstract

A technique has been developed for "staining" cytological preparations by indirect immunofluorescent methods that permits determination of the in situ distribution of chromosomal proteins. The method is particularly oriented to the use of polytene chromosome squashes from Drosophila salivary glands. Control experiments indicate that the fixation methods used allow little or no extraction or rearrangement of the chromosomal proteins. The results obtained demonstrate the specific in vivo chromosomal locations of nonhistone proteins purified from isolated chromatin. The technique is apparently capable of resolution at the level of the chromomere or band, the unit of genetic organization in Drosophila.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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