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. Author manuscript; available in PMC: 2013 May 29.
Published in final edited form as: Chembiochem. 2012 Apr 19;13(8):1112–1115. doi: 10.1002/cbic.201200091

Figure 4.

Figure 4

Pulse-chase assay to analyze poly(A) tail dynamics. (A) Experimental flow. Cells were labeled for two hours with EU or N6pA and chased for 2, 4, or 6 hr prior to cytoplasmic RNA extraction. Poly(A) tails of captured transcripts were then analyzed by northern blot using an oligo dT probe. (B) Data from an in vivo poly(A) tail dynamics assay.