Skip to main content
Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1976 Feb;73(2):554–558. doi: 10.1073/pnas.73.2.554

Effect of Xenopus laevis oocyte extract on supercoiled simian virus 40 DNA: formation of complex DNA.

D Gandini Attardi, G Martini, E Mattoccia, G P Tocchini-Valentini
PMCID: PMC335948  PMID: 174111

Abstract

A soluble cell-free extract derived from stage 6 Xenopus laevis oocytes is described. From supercoiled simian virus 40 DNA the extract produces nicked circles (having a single-strand scission), linear molecules of full unit size, shorter length fragments, and various forms of complex DNA.

Full text

PDF
554

Images in this article

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Francke B., Hunter T. In vitro polyoma DNA synthesis: requirement for cytoplasmic factors. J Virol. 1975 Jan;15(1):97–107. doi: 10.1128/jvi.15.1.97-107.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Germond J. E., Hirt B., Oudet P., Gross-Bellark M., Chambon P. Folding of the DNA double helix in chromatin-like structures from simian virus 40. Proc Natl Acad Sci U S A. 1975 May;72(5):1843–1847. doi: 10.1073/pnas.72.5.1843. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Gordon C. N., Rush M. G., Warner R. C. Complex replicative form molecules of bacteriophages phi X174 and S13 su105. J Mol Biol. 1970 Feb 14;47(3):495–503. doi: 10.1016/0022-2836(70)90317-7. [DOI] [PubMed] [Google Scholar]
  4. Gottesman S., Gottesman M. Excision of prophage lambda in a cell-free system. Proc Natl Acad Sci U S A. 1975 Jun;72(6):2188–2192. doi: 10.1073/pnas.72.6.2188. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Hirt B. Replicating molecules of polyoma virus DNA. J Mol Biol. 1969 Feb 28;40(1):141–144. doi: 10.1016/0022-2836(69)90302-7. [DOI] [PubMed] [Google Scholar]
  6. Hirt B. Selective extraction of polyoma DNA from infected mouse cell cultures. J Mol Biol. 1967 Jun 14;26(2):365–369. doi: 10.1016/0022-2836(67)90307-5. [DOI] [PubMed] [Google Scholar]
  7. Jaenisch R., Levine A. DNA replication in SV40-infected cells. V. Circular and catenated oligomers of SV40 DNA. Virology. 1971 Jun;44(3):480–493. doi: 10.1016/0042-6822(71)90361-8. [DOI] [PubMed] [Google Scholar]
  8. Kriegstein H. J., Hogness D. S. Mechanism of DNA replication in Drosophila chromosomes: structure of replication forks and evidence for bidirectionality. Proc Natl Acad Sci U S A. 1974 Jan;71(1):135–139. doi: 10.1073/pnas.71.1.135. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Laskey R. A., Gurdon J. B. Induction of polyoma DNA synthesis by injection into frog-egg cytoplasm. Eur J Biochem. 1973 Sep 3;37(3):467–471. doi: 10.1111/j.1432-1033.1973.tb03007.x. [DOI] [PubMed] [Google Scholar]
  10. Magnusson G., Winnacker E. L., Eliasson R., Reichard P. Replication of polyoma DNA in isolated nuclei. II. Evidence for semi-conservative replication. J Mol Biol. 1972 Dec 30;72(3):539–552. doi: 10.1016/0022-2836(72)90173-8. [DOI] [PubMed] [Google Scholar]
  11. Mulder C., Delius H. Specificity of the break produced by restricting endonuclease R 1 in Simian virus 40 DNA, as revealed by partial denaturation mapping. Proc Natl Acad Sci U S A. 1972 Nov;69(11):3215–3219. doi: 10.1073/pnas.69.11.3215. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Nash H. A. Integrative recombination of bacteriophage lambda DNA in vitro. Proc Natl Acad Sci U S A. 1975 Mar;72(3):1072–1076. doi: 10.1073/pnas.72.3.1072. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Rush M. G., Eason R., Vinograd J. Identification and properties of complex forms of SV40 DNA isolated from SV40-infected African Green monkey (BSC-1) cells. Biochim Biophys Acta. 1971 Feb 11;228(3):585–594. doi: 10.1016/0005-2787(71)90723-4. [DOI] [PubMed] [Google Scholar]
  14. Sakakibara Y., Tomizawa J. I. Replication of colicin E1 plasmid DNA in cell extracts. Proc Natl Acad Sci U S A. 1974 Mar;71(3):802–806. doi: 10.1073/pnas.71.3.802. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Schorderet-Slatkine S. Action of progesterone and related steroids on oocyte maturation in Xenopus laevis. An in vitro study. Cell Differ. 1972 Aug;1(3):179–189. doi: 10.1016/0045-6039(72)90027-9. [DOI] [PubMed] [Google Scholar]
  16. Tatò F., Gandini D. A., Tocchini-Valentini G. P. Major DNA polymerases common to different Xenopus laevis cell types. Proc Natl Acad Sci U S A. 1974 Sep;71(9):3706–3710. doi: 10.1073/pnas.71.9.3706. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Tocchini-Valentini G. P., Mahdavi V., Brown R., Crippa M. The synthesis of amplified ribosomal DNA. Cold Spring Harb Symp Quant Biol. 1974;38:551–558. doi: 10.1101/sqb.1974.038.01.059. [DOI] [PubMed] [Google Scholar]
  18. White M., Eason R. Nucleoprotein complexes in simian virus 40-infected cells. J Virol. 1971 Oct;8(4):363–371. doi: 10.1128/jvi.8.4.363-371.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Proceedings of the National Academy of Sciences of the United States of America are provided here courtesy of National Academy of Sciences

RESOURCES