Table 1.

Tumor suppressor genes upregulated by 5-Aza-CdR treatment in metastatic 4C11+ melanoma cell line

Genes upregulated after 5-Aza-CdR treatment	Function
Apod	Multifunctional transport and tissue homeostasis
Bst2	Immune response
Cdkn1c*	Cell cycle arrest
Fas	Apoptosis
Ifit1	Response to INFα
Itgae*	Cell adhesion
Socs2*	Negative regulation of JAK/STAT cascade

Genes related to apoptosis (Fas), cell cycle arrest (Cdkn1c), negative regulation of JAK/Stat cascade (Socs2), multifunctional transport and tissue homeostasis (Apod), cell adhesion (Itgae), IFN response (Ifit1) and immune response (Bst2) were found upregulated in metastatic 4C11+ melanoma cell line after 5-Aza-CdR treatment. Cells were cultured in RPMI medium with 10% FBS with or without 10 µM of 5-Aza-CdR for 48 h and gene expression profile was determined by microarray. The genes listed in this table were identified by SAM statistical analysis (FDR threshold at 5%).

^*means the presence of CpG island around transcription start site or near upstream site presumed by UCSC Genome Browser Bioinformatics (available online at genome.ucsc.edu/).