Hypoxia promotes TNFα production and metallopeptidase ADAM inhibitor blocks TNFα production by placental trophoblasts in culture. A: TNFα production in trophoblasts treated with CoCl2 at concentration of 100, 250, and 500μM for 48hrs. CoCl2 induced TNFα production is in a dose-dependent manner. Data are mean ± SE from 4 independent primary trophoblast culture experiments. B: TNFα production by trophoblasts treated with CoCl2 at different concentrations (0, 100, 250, and 500μM) in the presence or absence of a metallopeptidase inhibitor GM6001. GM6001 at 50μM concentration was used. Trophoblast production of TNFα induced by CoCl2 was significantly inhibited by GM6001. TNFα production by untreated cells is presented as 1 for each experiment. The data is presented as mean ± SE of fold changes for TNFα productions in CoCl2 treated cells vs. untreated controls Data are from 6 independent primary trophoblast culture experiments.