Abstract
The distribution of UV-induced DNA repair synthesis within chromatin was measured in confluent human fibroblasts that were pulse-labeled with [3H]dThd (10 or 90 min) immediately after irradiation and chased in nonradioactive medium for different time periods. Initially (i.e., at the end of the pulse period), most of the repair synthesis occurs in staphylococcal nuclease-sensitive regions. With increasing chase times the nucleotides inserted during repair synthesis become progressively more nuclease resistant. Gel electrophoresis data indicate that nuclease resistance is conferred on these nucleotides by their appearance in core DNA. The kinetics of this rearrangement process are biphasic: greater than 85% of the repair synthesis sites undergo rapid rearrangement (4--5 hr); the remaining sites ( less than 15%) rearrange much more slowly, if at all. The time courses of nucleosome rearrangement and repair synthesis are similar, suggesting that nucleosome rearrangement may be induced by the repair process or that the rate of repair synthesis may be regulated by nucleosome rearrangement.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Cleaver J. E. Nucleosome structure controls rates of excision repair in DNA of human cells. Nature. 1977 Dec 1;270(5636):451–453. doi: 10.1038/270451a0. [DOI] [PubMed] [Google Scholar]
- Crémisi C., Pignatti P. F., Yaniv M. Random location and absence of movement of the nucleosomes on SV 40 nucleoprotein complex isolated from infected cells. Biochem Biophys Res Commun. 1976 Dec 6;73(3):548–554. doi: 10.1016/0006-291x(76)90845-7. [DOI] [PubMed] [Google Scholar]
- Elgin S. C., Weintraub H. Chromosomal proteins and chromatin structure. Annu Rev Biochem. 1975;44:725–774. doi: 10.1146/annurev.bi.44.070175.003453. [DOI] [PubMed] [Google Scholar]
- Kornberg R. D. Structure of chromatin. Annu Rev Biochem. 1977;46:931–954. doi: 10.1146/annurev.bi.46.070177.004435. [DOI] [PubMed] [Google Scholar]
- Kovacic R. T., van Holde K. E. Sedimentation of homogeneous double-strand DNA molecules. Biochemistry. 1977 Apr 5;16(7):1490–1498. doi: 10.1021/bi00626a038. [DOI] [PubMed] [Google Scholar]
- McKnight S. L., Miller O. L., Jr Electron microscopic analysis of chromatin replication in the cellular blastoderm Drosophila melanogaster embryo. Cell. 1977 Nov;12(3):795–804. doi: 10.1016/0092-8674(77)90278-1. [DOI] [PubMed] [Google Scholar]
- Nichols W. W., Murphy D. G., Cristofalo V. J., Toji L. H., Greene A. E., Dwight S. A. Characterization of a new human diploid cell strain, IMR-90. Science. 1977 Apr 1;196(4285):60–63. doi: 10.1126/science.841339. [DOI] [PubMed] [Google Scholar]
- Scheer U. Changes of nucleosome frequency in nucleolar and non-nucleolar chromatin as a function of transcription: an electron microscopic study. Cell. 1978 Mar;13(3):535–549. doi: 10.1016/0092-8674(78)90327-6. [DOI] [PubMed] [Google Scholar]
- Seale R. L. Assembly of DNA and protein during replication in HeLa cells. Nature. 1975 May 15;255(5505):247–249. doi: 10.1038/255247a0. [DOI] [PubMed] [Google Scholar]
- Seale R. L. Studies on the mode of segregation of histone nu bodies during replication in HeLa cells. Cell. 1976 Nov;9(3):423–429. doi: 10.1016/0092-8674(76)90087-8. [DOI] [PubMed] [Google Scholar]
- Smerdon M. J., Tlsty T. D., Lieberman M. W. Distribution of ultraviolet-induced DNA repair synthesis in nuclease sensitive and resistant regions of human chromatin. Biochemistry. 1978 Jun 13;17(12):2377–2386. doi: 10.1021/bi00605a020. [DOI] [PubMed] [Google Scholar]
- Weintraub H. The assembly of newly replicated DNA into chromatin. Cold Spring Harb Symp Quant Biol. 1974;38:247–256. doi: 10.1101/sqb.1974.038.01.028. [DOI] [PubMed] [Google Scholar]