Abstract
A soluble adenovirus DNA replication system has been prepared in extracts of HeLa cells infected with the temperature-sensitive mutant H5ts125. These nuclear extracts synthesize full-sized viral DNA at 30 degrees but are temperature sensitive in this function at 38 degrees. A 72,000-dalton DNA-binding protein purified from cells infected with wild-type virus complements the temperature-sensitive defect and restores viral DNA replication to 74% of normal values.
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Selected References
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- Brison O., Kedinger C., Wilhelm J. Enzymatic properties of viral replication complexes isolated from adenovirus type 2-infected HeLa cell nuclei. J Virol. 1977 Nov;24(2):423–435. doi: 10.1128/jvi.24.2.423-435.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Chow L. T., Roberts J. M., Lewis J. B., Broker T. R. A map of cytoplasmic RNA transcripts from lytic adenovirus type 2, determined by electron microscopy of RNA:DNA hybrids. Cell. 1977 Aug;11(4):819–836. doi: 10.1016/0092-8674(77)90294-x. [DOI] [PubMed] [Google Scholar]
- EAGLE H. Amino acid metabolism in mammalian cell cultures. Science. 1959 Aug 21;130(3373):432–437. doi: 10.1126/science.130.3373.432. [DOI] [PubMed] [Google Scholar]
- Edenberg H. J., Waqar M. A., Huberman J. A. Subnuclear systems for synthesis of simian virus 40 DNA in vitro. Proc Natl Acad Sci U S A. 1976 Dec;73(12):4392–4396. doi: 10.1073/pnas.73.12.4392. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Ginsberg H. S., Ensinger M. J., Kauffman R. S., Mayer A. J., Lundholm U. Cell transformation: a study of regulation with types 5 and 12 adenovirus temperature-sensitive mutants. Cold Spring Harb Symp Quant Biol. 1975;39(Pt 1):419–426. doi: 10.1101/sqb.1974.039.01.054. [DOI] [PubMed] [Google Scholar]
- Ginsberg H. S., Young C. S. Genetics of adenoviruses. Adv Cancer Res. 1976;23:91–130. doi: 10.1016/s0065-230x(08)60544-8. [DOI] [PubMed] [Google Scholar]
- Horwitz M. S. Intermediates in the synthesis of type 2 adenovirus deoxyribonucleic acid. J Virol. 1971 Nov;8(5):675–683. doi: 10.1128/jvi.8.5.675-683.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Kaplan L. M., Kelinman R. E., Horwitz M. S. Replication of adenovirus type 2 DNA in vitro. Proc Natl Acad Sci U S A. 1977 Oct;74(10):4425–4429. doi: 10.1073/pnas.74.10.4425. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Kedinger C., Brison O., Perrin F., Wilhelm J. Structural analysis of viral replicative intermediates isolated from adenovirus type 2-infected HeLa cell nuclei. J Virol. 1978 May;26(2):364–379. doi: 10.1128/jvi.26.2.364-379.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
- LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
- Leibowitz J., Horwitz M. S. Synthesis and assembly of adenovirus polypeptides. III. Reversible inhibition of hexon assembly in adenovirus type 5 temperature-sensitive mutants. Virology. 1975 Jul;66(1):10–24. doi: 10.1016/0042-6822(75)90175-0. [DOI] [PubMed] [Google Scholar]
- Maizel J. V., Jr, White D. O., Scharff M. D. The polypeptides of adenovirus. I. Evidence for multiple protein components in the virion and a comparison of types 2, 7A, and 12. Virology. 1968 Sep;36(1):115–125. doi: 10.1016/0042-6822(68)90121-9. [DOI] [PubMed] [Google Scholar]
- Masker W. E., Richardson C. C. Bacteriophage T7 deoxyribonucleic acid replication in vitro VI. Synthesis of biologically active T7 DNA. J Mol Biol. 1976 Feb 5;100(4):557–567. doi: 10.1016/s0022-2836(76)80045-9. [DOI] [PubMed] [Google Scholar]
- Russel W. C., Newman C., Williams J. F. Characterization of temperature-sensitive mutants of adenovirus type 5--serology. J Gen Virol. 1972 Dec;17(3):265–279. doi: 10.1099/0022-1317-17-3-265. [DOI] [PubMed] [Google Scholar]
- Schaller H., Nüsslein C., Bonhoeffer F. J., Kurz C., Nietzschmann I. Affinity chromatography of DNA-binding enzymes on single-stranded DNA-agarose columns. Eur J Biochem. 1972 Apr 24;26(4):474–481. doi: 10.1111/j.1432-1033.1972.tb01789.x. [DOI] [PubMed] [Google Scholar]
- Shimojo H., Shiroki K., Yamaguchi K. The viral DNA replication machinery of adenovirus 12. Cold Spring Harb Symp Quant Biol. 1975;39(Pt 1):533–538. doi: 10.1101/sqb.1974.039.01.065. [DOI] [PubMed] [Google Scholar]
- Slater M. L., Ozer H. L. Temperature-sensitive mutants of Balb/3T3 cells: description of a mutant affected in cellular and polyoma virus DNA synthesis. Cell. 1976 Feb;7(2):289–295. doi: 10.1016/0092-8674(76)90028-3. [DOI] [PubMed] [Google Scholar]
- Su R. T., DePamphilis M. L. In vitro replication of simian virus 40 DNA in a nucleoprotein complex. Proc Natl Acad Sci U S A. 1976 Oct;73(10):3466–3470. doi: 10.1073/pnas.73.10.3466. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Sugawara K., Gilead Z., Green M. Purification and molecular characterization of adenovirus type 2 DNA-binding protein. J Virol. 1977 Jan;21(1):338–346. doi: 10.1128/jvi.21.1.338-346.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Suzuki E., Shimojo H. Temperature-sensitive formation of the DNA replication complex in adenovirus 31-infected cells. J Virol. 1974 Feb;13(2):538–540. doi: 10.1128/jvi.13.2.538-540.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Tegtmeyer P. Simian virus 40 deoxyribonucleic acid synthesis: the viral replicon. J Virol. 1972 Oct;10(4):591–598. doi: 10.1128/jvi.10.4.591-598.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Van Der Vliet P. C., Levine A. J., Ensinger M. J., Ginsberg H. S. Thermolabile DNA binding proteins from cells infected with a temperature-sensitive mutant of adenovrius defective in viral DNA synthesis. J Virol. 1975 Feb;15(2):348–354. doi: 10.1128/jvi.15.2.348-354.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Van der Vliet P. C., Zandberg J., Jansz H. S. Evidence for a function of the adenovirus DNA-binding protein in initiation in DNA synthesis as well as in elongation of nascent DNA chains. Virology. 1977 Jul 1;80(1):98–110. doi: 10.1016/0042-6822(77)90383-x. [DOI] [PubMed] [Google Scholar]
- Vliet P. C., Sussenbach J. S. An adenovirus type 5 gene function required for initiation of viral DNA replication. Virology. 1975 Oct;67(2):415–426. doi: 10.1016/0042-6822(75)90443-2. [DOI] [PubMed] [Google Scholar]
- Wilkie N. M., Ustacelebi S., Williams J. F. Characterization of temperature-sensitive mutants of adenovirus type 5: nucleic acid synthesis. Virology. 1973 Feb;51(2):499–503. doi: 10.1016/0042-6822(73)90450-9. [DOI] [PubMed] [Google Scholar]
- Winnacker E. L., Magnusson G., Reichard P. Replication of polyoma DNA in isolated nuclei. I. Characterization of the system from mouse fibroblast 3T6 cells. J Mol Biol. 1972 Dec 30;72(3):523–537. doi: 10.1016/0022-2836(72)90172-6. [DOI] [PubMed] [Google Scholar]
- Yamashita T., Arens M., Green M. Adenovirus deoxyribonucleic acid replication. Isolation of a soluble replication system and analysis of the in vitro DNA product. J Biol Chem. 1977 Nov 25;252(22):7940–7946. [PubMed] [Google Scholar]
- van der Vliet P. C., Levine A. J. DNA-binding proteins specific for cells infected by adenovirus. Nat New Biol. 1973 Dec 12;246(154):170–174. doi: 10.1038/newbio246170a0. [DOI] [PubMed] [Google Scholar]
- van der Vliet P. C., Sussenbach J. S. The mechanism of adenovirus-DNA synthesis in isolated nuclei. Eur J Biochem. 1972 Nov 7;30(3):584–592. doi: 10.1111/j.1432-1033.1972.tb02130.x. [DOI] [PubMed] [Google Scholar]