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. 1978 Sep;75(9):4484–4488. doi: 10.1073/pnas.75.9.4484

Actin may be present on the lymphocyte surface.

M J Owen, J Auger, B H Barber, A J Edwards, F S Walsh, M J Crumpton
PMCID: PMC336140  PMID: 309133

Abstract

Lymphocytes were assessed for the presence of surface actin and myosin by lactoperoxidase-catalyzed iodination and indirect immunofluorescence using antisera against purified pig skeletal muscle actin and pig smooth muscle myosin. Sodium dodecyl sulfate/polyacrylamide gel electrophoresis of 125I-labeled pig, mouse, and human B lymphocytes revealed an intense radioactive band of 43,000 molecular weight, whereas pig and mouse T lymphocytes gave a much less intense band. This band comigrated with actin, was nonglycosylated as judged by lack of binding to lentil lectin-Sepharose, was bound specifically by myosin fibers, and could be distinguished from a polypeptide of similar mobility derived from the major histocompatibility antigens. These results suggest that actin is present on the surface of B lymphocytes and, to a lesser extent, on T lymphocytes. Pig, mouse, and human Ig-bearing cells were stained by antiactin and antimyosin antisera, as judged by indirect immunofluorescence, whereas non-Ig-bearing cells were not stained. Antibody binding, however, was depleted by adsorbing the antisera with Ig-Sepharose. It was concluded that the immunofluorescence results are misleading and reflect the presence of antibodies that crossreact with Ig.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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