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. 1978 Sep;75(9):4544–4548. doi: 10.1073/pnas.75.9.4544

Isolated microvessels: the blood-brain barrier in vitro.

J T Hjelle, J Baird-Lambert, G Cardinale, S Specor, S Udenfriend
PMCID: PMC336153  PMID: 279933

Abstract

Isolated bovine retinal and brain microvessels, exhibiting a patent lumen, were used to study the contribution of the microvasculature to the blood-brain and blood-retina barriers. The diffusion marker, sucrose, was taken up slowly by the isolated microvessels in contrast to leucine, tyrosine, and valine which were taken up at a considerably faster rate. Uptake of leucine was temperature dependent but resistant to inhibition by ouabain and sodium azide. The large neutral amino acids exhibited stereospecificity and cross-competition for uptake by the isolated microvessels. The apparent Kms for uptake for tyrosine, leucine, and valine were III micron,133 micron, and 500 micron, respectively.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Battistin L., Grynbaum A., Lajtha A. The uptake of various amino acids by the mouse brain in vivo. Brain Res. 1971 Jun 4;29(1):85–99. doi: 10.1016/0006-8993(71)90419-7. [DOI] [PubMed] [Google Scholar]
  2. Baños G., Daniel P. M., Moorhouse S. R., Pratt O. E. The influx of amino acids into the brain of the rat in vivo: the essential compared with some non-essential amino acids. Proc R Soc Lond B Biol Sci. 1973 Feb 27;183(1070):59–70. doi: 10.1098/rspb.1973.0004. [DOI] [PubMed] [Google Scholar]
  3. Bertler A., Falck B., Owman C., Rosengrenn E. The localization of monoaminergic blood-brain barrier mechanisms. Pharmacol Rev. 1966 Mar;18(1):369–385. [PubMed] [Google Scholar]
  4. Brendel K., Meezan E., Carlson E. C. Isolated brain microvessels: a purified, metabolically active preparation from bovine cerebral cortex. Science. 1974 Sep 13;185(4155):953–955. doi: 10.1126/science.185.4155.953. [DOI] [PubMed] [Google Scholar]
  5. Brender J., Andersen P. E., Rafaelsen O. J. Blood-brain transfer of d-glucose, l-leucine, and l-tryptophan in the rat. Acta Physiol Scand. 1975 Apr;93(4):490–499. doi: 10.1111/j.1748-1716.1975.tb05840.x. [DOI] [PubMed] [Google Scholar]
  6. Brightman M. W. Morphology of blood-brain interfaces. Exp Eye Res. 1977;25 (Suppl):1–25. doi: 10.1016/s0014-4835(77)80008-0. [DOI] [PubMed] [Google Scholar]
  7. Brightman M. W., Reese T. S. Junctions between intimately apposed cell membranes in the vertebrate brain. J Cell Biol. 1969 Mar;40(3):648–677. doi: 10.1083/jcb.40.3.648. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. CHIRIGOS M. A., GREENGARD P., UDENFRIEND S. Uptake of tyrosine by rat brain in vivo. J Biol Chem. 1960 Jul;235:2075–2079. [PubMed] [Google Scholar]
  9. Christensen H. N., Oxender D. L., Liang M., Vatz K. A. The use of N-methylation to direct route of mediated transport of amino acids. J Biol Chem. 1965 Sep;240(9):3609–3616. [PubMed] [Google Scholar]
  10. Constantinidis J., Gaillard J. M., Geissbuhler F., Tissot R. Passage into the rat brain of dopa and dopamine injected into the lateral ventricle. Br J Pharmacol. 1971 Sep;43(1):32–38. doi: 10.1111/j.1476-5381.1971.tb07154.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. GUROFF G., UDENFRIEND S. Studies on aromatic amino acid uptake by rat brain in vivo. Uptake of phenylalanine and of tryptophan; inhibition and stereoselectivity in the uptake of tyrosine by brain and muscle. J Biol Chem. 1962 Mar;237:803–806. [PubMed] [Google Scholar]
  12. Goldstein G. W., Wolinsky J. S., Csejtey J., Diamond I. Isolation of metabolically active capillaries from rat brain. J Neurochem. 1975 Nov;25(5):715–717. doi: 10.1111/j.1471-4159.1975.tb04395.x. [DOI] [PubMed] [Google Scholar]
  13. LAJTHA A., TOTH J. The brain barrier system--II. Uptake and transport of amino acids by the brain. J Neurochem. 1961 Dec;8:216–225. doi: 10.1111/j.1471-4159.1961.tb13545.x. [DOI] [PubMed] [Google Scholar]
  14. Lai F. M., Spector S. Brain and vascular monoamine oxidase activity in the deoxycorticosterone-salt hypertensive rat. Br J Pharmacol. 1977 Mar;59(3):393–395. doi: 10.1111/j.1476-5381.1977.tb08391.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Lai F. M., Udenfriend S., Spector S. Presence of norepinephrine and related enzymes in isolated brain microvessels. Proc Natl Acad Sci U S A. 1975 Nov;72(11):4622–4625. doi: 10.1073/pnas.72.11.4622. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Levin E. Are the terms blood-brain barrier and brain capillary permeability synonymous? Exp Eye Res. 1977;25 (Suppl):191–199. doi: 10.1016/s0014-4835(77)80017-1. [DOI] [PubMed] [Google Scholar]
  17. Meezan E., Brendel K., Carlson E. C. Isolation of a purified preparation of metabolically active retinal blood vessels. Nature. 1974 Sep 6;251(5470):65–67. doi: 10.1038/251065a0. [DOI] [PubMed] [Google Scholar]
  18. ORLOWSKI M., MEISTER A. ISOLATION OF GAMMA-GLUTAMYL TRANSPEPTIDASE FROM HOG KIDNEY. J Biol Chem. 1965 Jan;240:338–347. [PubMed] [Google Scholar]
  19. Oldendorf W. H. Stereospecificity of blood-brain barrier permeability to amino acids. Am J Physiol. 1973 Apr;224(4):967–969. doi: 10.1152/ajplegacy.1973.224.4.967. [DOI] [PubMed] [Google Scholar]
  20. Oldendorf W. H., Szabo J. Amino acid assignment to one of three blood-brain barrier amino acid carriers. Am J Physiol. 1976 Jan;230(1):94–98. doi: 10.1152/ajplegacy.1976.230.1.94. [DOI] [PubMed] [Google Scholar]
  21. Oldendorf W. H. The blood-brain barrier. Exp Eye Res. 1977;25 (Suppl):177–190. doi: 10.1016/s0014-4835(77)80016-x. [DOI] [PubMed] [Google Scholar]
  22. Orlowski M., Sessa G., Green J. P. Gamma-glutamyl transpeptidase in brain capillaries: possible site of a blood-brain barrier for amino acids. Science. 1974 Apr 5;184(4132):66–68. doi: 10.1126/science.184.4132.66. [DOI] [PubMed] [Google Scholar]
  23. Owman C., Rosengren E. Dopamine formation in brain capillaries--an enzymic blood-brain barrier mechanism. J Neurochem. 1967 May;14(5):547–550. doi: 10.1111/j.1471-4159.1967.tb09553.x. [DOI] [PubMed] [Google Scholar]
  24. Pardridge W. M., Oldendorf W. H. Kinetic analysis of blood-brain barrier transport of amino acids. Biochim Biophys Acta. 1975 Aug 5;401(1):128–136. doi: 10.1016/0005-2736(75)90347-8. [DOI] [PubMed] [Google Scholar]
  25. Raviola G. The structural basis of the blood-ocular barriers. Exp Eye Res. 1977;25 (Suppl):27–63. doi: 10.1016/s0014-4835(77)80009-2. [DOI] [PubMed] [Google Scholar]
  26. Sampath S. S., Neff N. H. The elimination of 5-hydroxyindoleacetic acid from cerebrospinal fluid: characteristics of the acid transport system of the choroid plexus. J Pharmacol Exp Ther. 1974 Feb;188(2):410–414. [PubMed] [Google Scholar]
  27. Sershen H., Lajtha A. Capillary transport of amino acids in the developing brain. Exp Neurol. 1976 Nov;53(2):465–474. doi: 10.1016/0014-4886(76)90086-8. [DOI] [PubMed] [Google Scholar]
  28. Spector S., Lai F. M., Ooshima A., Cardinale G., Udenfriend S. Relation of catecholamines to collagen synthesis. Ann N Y Acad Sci. 1976;275:114–116. doi: 10.1111/j.1749-6632.1976.tb43345.x. [DOI] [PubMed] [Google Scholar]
  29. Stein S., Böhlen P., Stone J., Dairman W., Udenfriend S. Amino acid analysis with fluorescamine at the picomole level. Arch Biochem Biophys. 1973 Mar;155(1):202–212. doi: 10.1016/s0003-9861(73)80022-0. [DOI] [PubMed] [Google Scholar]
  30. Wade L. A., Katzman R. Synthetic amino acids and the nature of L-DOPA transport at the blood-brain barrier. J Neurochem. 1975 Dec;25(6):837–842. doi: 10.1111/j.1471-4159.1975.tb04415.x. [DOI] [PubMed] [Google Scholar]
  31. Yudilevich D. L., De Rose N., Sepúlveda F. V. Facilitated transport of amino acids through the blood-brain barrier of the dog studied in a single capillary circulation. Brain Res. 1972 Sep 29;44(2):569–578. doi: 10.1016/0006-8993(72)90319-8. [DOI] [PubMed] [Google Scholar]

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