Abstract
The serological properties of the gag gene products p15 and p12 of N- and B-tropic viruses of C57BL mice have been examined. Although these viruses were serologically identical by competition assays for proteins gp71 and p30, they were readily distinguishable in competition assays for proteins p15 and p12. Two isolates of N-tropic viruses had p12s serologically indistinguishable from AKR murine leukemia virus p12, while two B-tropic isolates had distinctly different p12s. The latter p12s were serologically indistinguishable from the p12 purified from the B-tropic radiation leukemia virus (RadLV)/VL-3. Moreover, this p12 was indistinguishable from the p12 of the endogenous C57BL/Ka xenotropic virus. Similarly, the p15s of the B-tropic viruses were serologically distinct from the AKR murine leukemia virus type of p15, as was the p15 of one C57BL N-tropic virus, whilc another N-tropic isolate had a p15 identical to the AKR murine leukemia virus p15. These results are interprered to suggest that the endogenous N-tropic virus of C57BL mice undergoes recombination with the endogenous, xenotropic virus and that this mechanism is involved in the generation of B-tropic viruses in C57BL mice.
Full text
PDF




Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Aaronson S. A., Stephenson J. R. Independent segregation of loci for activation of biologically distinguishable RNA C-type viruses in mouse cells. Proc Natl Acad Sci U S A. 1973 Jul;70(7):2055–2058. doi: 10.1073/pnas.70.7.2055. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Barbacid M., Stephenson J. R., Aaronson S. A. gag Gene of mammalian type-C RNA tumour viruses. Nature. 1976 Aug 12;262(5569):554–559. doi: 10.1038/262554a0. [DOI] [PubMed] [Google Scholar]
- Blair D. G. Genetic recombination between avian leukosis and sarcoma viruses. Experimental variables and the frequencies of recombination. Virology. 1977 Apr;77(2):534–544. doi: 10.1016/0042-6822(77)90479-2. [DOI] [PubMed] [Google Scholar]
- Callahan R., Benveniste R. E., Lieber M. M., Todaro G. J. Nucleic acid homology of murine type-C viral genes. J Virol. 1974 Dec;14(6):1394–1403. doi: 10.1128/jvi.14.6.1394-1403.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Declève A., Lieberman M., Ihle J. N., Kaplan H. S. Biological and serological characterization of radiation leukemia virus. Proc Natl Acad Sci U S A. 1976 Dec;73(12):4675–4679. doi: 10.1073/pnas.73.12.4675. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Declève A., Lieberman M., Kaplan H. S. In vivo interaction between RNA viruses isolated from the C57BL/Ka strain of mice. Virology. 1977 Sep;81(2):270–283. doi: 10.1016/0042-6822(77)90144-1. [DOI] [PubMed] [Google Scholar]
- Declève A., Niwa O., Gelmann E., Kaplan H. S. Replication kinetics of N- and B-tropic murine leukemia viruses on permissive and nonpermissive cells in vitro. Virology. 1975 Jun;65(2):320–332. doi: 10.1016/0042-6822(75)90038-0. [DOI] [PubMed] [Google Scholar]
- Declève A., Niwa O., Hilgers J., Kaplan H. S. An improved murine leukemia virus immunofluorescence assay. Virology. 1974 Feb;57(2):491–502. doi: 10.1016/0042-6822(74)90188-3. [DOI] [PubMed] [Google Scholar]
- GREENWOOD F. C., HUNTER W. M., GLOVER J. S. THE PREPARATION OF I-131-LABELLED HUMAN GROWTH HORMONE OF HIGH SPECIFIC RADIOACTIVITY. Biochem J. 1963 Oct;89:114–123. doi: 10.1042/bj0890114. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Gelmann E. P., Declève A., Kaplan H. S. Biological and biochemical differences among ecotropic C-type RNA viral isolates chemically induced from C57BL/KA mouse embryo cells in vitro. Virology. 1978 Mar;85(1):198–210. doi: 10.1016/0042-6822(78)90424-5. [DOI] [PubMed] [Google Scholar]
- Hartley J. W., Rowe W. P. Clonal cells lines from a feral mouse embryo which lack host-range restrictions for murine leukemia viruses. Virology. 1975 May;65(1):128–134. doi: 10.1016/0042-6822(75)90013-6. [DOI] [PubMed] [Google Scholar]
- Hartley J. W., Rowe W. P., Huebner R. J. Host-range restrictions of murine leukemia viruses in mouse embryo cell cultures. J Virol. 1970 Feb;5(2):221–225. doi: 10.1128/jvi.5.2.221-225.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Hartley J. W., Wolford N. K., Old L. J., Rowe W. P. A new class of murine leukemia virus associated with development of spontaneous lymphomas. Proc Natl Acad Sci U S A. 1977 Feb;74(2):789–792. doi: 10.1073/pnas.74.2.789. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Hopkins N., Schindler J., Hynes R. Six-NB-tropic murine leukemia viruses derived from a B-tropic virus of BALB/c have altered p30. J Virol. 1977 Jan;21(1):309–318. doi: 10.1128/jvi.21.1.309-318.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Ihle J. N., Denny T. P., Bolognesi D. P. Purification and serological characterization of the major envelope glycoprotein from AKR murine leukemia virus and its reactivity with autogenous immune sera from mice. J Virol. 1976 Mar;17(3):727–736. doi: 10.1128/jvi.17.3.727-736.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Ihle J. N., Joseph D. R. Genetic analysis of the endogenous C3H murine leukemia virus genome: evidence for one locus unlinked to the endogenous murine leukemia virus genome of C57BL/6 mice. Virology. 1978 Jun 15;87(2):298–306. doi: 10.1016/0042-6822(78)90135-6. [DOI] [PubMed] [Google Scholar]
- Peters R. L., Spahn G. J., Rabstein L. S., Kelloff G. J., Huebner R. J. Murine C-type RNA virus from spontaneous neoplasms: in vitro host range and oncogenic potential. Science. 1973 Aug 17;181(4100):665–667. doi: 10.1126/science.181.4100.665. [DOI] [PubMed] [Google Scholar]
- Pincus T., Hartley J. W., Rowe W. P. A major genetic locus affecting resistance to infection with murine leukemia viruses. I. Tissue culture studies of naturally occurring viruses. J Exp Med. 1971 Jun 1;133(6):1219–1233. doi: 10.1084/jem.133.6.1219. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Pincus T., Rowe W. P., Lilly F. A major genetic locus affecting resistance to infection with murine leukemia viruses. II. Apparent identity to a major locus described for resistance to friend murine leukemia virus. J Exp Med. 1971 Jun 1;133(6):1234–1241. doi: 10.1084/jem.133.6.1234. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Robbins K. C., Cabradilla C. D., Stephenson J. R., Aaronson S. A. Segregation of genetic information for a B-tropic leukemia virus with the structural locus for BALB:virus-1. Proc Natl Acad Sci U S A. 1977 Jul;74(7):2953–2957. doi: 10.1073/pnas.74.7.2953. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Robbins K. C., Cabradilla C. D., Stephenson J. R., Aaronson S. A. Segregation of genetic information for a B-tropic leukemia virus with the structural locus for BALB:virus-1. Proc Natl Acad Sci U S A. 1977 Jul;74(7):2953–2957. doi: 10.1073/pnas.74.7.2953. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Rowe W. P. Genetic factors in the natural history of murine leukemia virus infection: G. H. A. Clowes Memorial Lecture. Cancer Res. 1973 Dec;33(12):3061–3068. [PubMed] [Google Scholar]
- Rowe W. P., Hartley J. W., Bremner T. Genetic mapping of a murine leukemia virus-inducing locus of AKR mice. Science. 1972 Nov 24;178(4063):860–862. doi: 10.1126/science.178.4063.860. [DOI] [PubMed] [Google Scholar]
- Rowe W. P., Humphrey J. B., Lilly F. A major genetic locus affecting resistance to infection with murine leukemia viruses. 3. Assignment of the Fv-1 locus to linkage group 8 of the mouse. J Exp Med. 1973 Mar 1;137(3):850–853. doi: 10.1084/jem.137.3.850. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Rowe W. P., Pugh W. E., Hartley J. W. Plaque assay techniques for murine leukemia viruses. Virology. 1970 Dec;42(4):1136–1139. doi: 10.1016/0042-6822(70)90362-4. [DOI] [PubMed] [Google Scholar]
- Schindler J., Hynes R., Hopkins N. Evidence for recombination between N- and B-tropic murine leukemia viruses: analysis of three virion proteins by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. J Virol. 1977 Sep;23(3):700–700. doi: 10.1128/jvi.23.3.700-.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Staal S. P., Hartley J. W., Rowe W. P. Isolation of transforming murine leukemia viruses from mice with a high incidence of spontaneous lymphoma. Proc Natl Acad Sci U S A. 1977 Jul;74(7):3065–3067. doi: 10.1073/pnas.74.7.3065. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Vogt P. K. Genetically stable reassortment of markers during mixed infection with avian tumor viruses. Virology. 1971 Dec;46(3):947–952. doi: 10.1016/0042-6822(71)90093-6. [DOI] [PubMed] [Google Scholar]