Figure 4.
Specific killing of induced Vero-219 cells by 2014-PE38 as measured by the infectious virus release assay. Vero-219 cells were induced by BacK50 plus sodium butyrate and treated for 72 h with the indicated concentrations of 2014-PE38 (solid circles) or the control proteins BL22 (open squares) or 2014-PE38E553 (open triangles). The supernatants were harvested and assayed for infection of fresh 293 cells to measure the infectious KSHV-r129 produced from the activated cells. The amount of infectious virus produced from activated Vero-219 cells with no addition was set as 100%. Each data point represents the mean of duplicate culture wells with error bars as standard deviation.