Figure 5. Nr-CAM and Plexin-A1 in crossed RGCs mediate effects of Sema6D on RGC outgrowth.

(A) Outgrowth of Plexin-A1^−/−;Nr-CAM^−/− DT explants is reduced on WT chiasm cells compared to WT, Plexin-A1^−/− single or Nr-CAM^−/− DT explants. (B) Summary of results in Figure 5A. Note that outgrowth of Plexin-A1^−/− or Nr-CAM^−/− DT explants are only partially reduced, but outgrowth of Plexin-A1^−/−;Nr-CAM^−/− DT explants are further reduced on WT chiasm cells. (C) Quantification of Nr-CAM^−/−, Plexin-A1^−/− and Plexin-A1^−/−;Nr-CAM^−/− outgrowth from DT retinal explants on Sema6D⁺ HEK cells. Note that Sema6D⁺ HEK cells only partially inhibit outgrowth of Nr-CAM^−/− or Plexin-A1^−/− DT explants, but neurite outgrowth is similar to controls in Plexin-A1^−/−;Nr-CAM^−/− DT explants. (D) Outgrowth of Nr-CAM^−/−, Plexin-A1^−/− and Plexin-A1^−/−;Nr-CAM^−/− DT retinal outgrowth on Sema6D⁺/Nr-CAM⁺ and Plexin-A1⁺ HEK cells. (E) Summary of results in Figures 5C and 5D. Note that Plexin-A1^−/−;Nr-CAM^−/− DT axons grew no differently on Sema6D⁺ HEK cells or on Sema6D⁺/Nr-CAM⁺ and Plexin-A1⁺ HEK cells than on HEK cells expressing empty vector. (n) = number of explants for each condition, * p<0.01