Skip to main content
NCBI home page
Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1978 Oct;75(10):5043–5047. doi: 10.1073/pnas.75.10.5043

Inducers of DNA synthesis present during mitosis of mammalian cells lacking G1 and G2 phases.

P N Rao, B A Wilson, P S Sunkara
PMCID: PMC336259  PMID: 283413

Abstract

The cell cycle analysis of Chinese hamster lung fibroblast V79-8 line by the premature chromosome condensation method has confirmed the absence of measurable G1 and G2 periods. Sendai virus-mediated fusion of mitotic V79-8 cells with G1 phase HeLa cells resulted in the induction of both DNA synthesis and premature chromosome condensation in the latter, indicating the presence of the inducers of DNA synthesis above the critical level not only throughout S phase, as it is in HeLa, but also during mitosis of V79-8 cells. No initiation of DNA synthesis was observed when G1 phase HeLa cells were fused with mitotic CHO cells. These results indicate that the presence or absence of a G1 period in the cell cycle depends on the levels of the inducers of DNA synthesis present in the cell during mitosis.

Full text

PDF
5043

Images in this article

5044Image
on p.5044
5044Image
on p.5044
5045Image
on p.5045
5045Image
on p.5045
5045Image
on p.5045
5045Image
on p.5045

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. De Terra N. MACRONUCLEAR DNA SYNTHESIS IN Stentor: REGULATION BY A CYTOPLASMIC INITIATOR. Proc Natl Acad Sci U S A. 1967 Mar;57(3):607–614. doi: 10.1073/pnas.57.3.607. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Graves J. A. DNA synthesis in heterokaryons formed by fusion of mammalian cells from different species. Exp Cell Res. 1972 Jun;72(2):393–403. doi: 10.1016/0014-4827(72)90007-9. [DOI] [PubMed] [Google Scholar]
  3. Gurdon J. B. On the origin and persistence of a cytoplasmic state inducing nuclear DNA synthesis in frogs' eggs. Proc Natl Acad Sci U S A. 1967 Aug;58(2):545–552. doi: 10.1073/pnas.58.2.545. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Hittelman W. N., Rao P. N. Mapping G1 phase by the structural morphology of the prematurely condensed chromosomes. J Cell Physiol. 1978 Jun;95(3):333–341. doi: 10.1002/jcp.1040950311. [DOI] [PubMed] [Google Scholar]
  5. Liskay R. M. Absence of a measurable G2 phase in two Chinese hamster cell lines. Proc Natl Acad Sci U S A. 1977 Apr;74(4):1622–1625. doi: 10.1073/pnas.74.4.1622. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Pederson T. Chromatin structure and the cell cycle. Proc Natl Acad Sci U S A. 1972 Aug;69(8):2224–2228. doi: 10.1073/pnas.69.8.2224. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Pederson T., Robbins E. Chromatin structure and the cell division cycle. Actinomycin binding in synchronized HeLa cells. J Cell Biol. 1972 Nov;55(2):322–327. doi: 10.1083/jcb.55.2.322. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. RAO P. N., ENGELBERG J. HELA CELLS: EFFECTS OF TEMPERATURE ON THE LIFE CYCLE. Science. 1965 May 21;148(3673):1092–1094. doi: 10.1126/science.148.3673.1092. [DOI] [PubMed] [Google Scholar]
  9. Rao P. N., Hittelman W. N., Wilson B. A. Mammalian cell fusion. VI. Regulation of mitosis in binucleate HeLa cells. Exp Cell Res. 1975 Jan;90(1):40–46. doi: 10.1016/0014-4827(75)90354-7. [DOI] [PubMed] [Google Scholar]
  10. Rao P. N., Johnson R. T. Mammalian cell fusion: studies on the regulation of DNA synthesis and mitosis. Nature. 1970 Jan 10;225(5228):159–164. doi: 10.1038/225159a0. [DOI] [PubMed] [Google Scholar]
  11. Rao P. N. Mitotic synchrony in mammalian cells treated with nitrous oxide at high pressure. Science. 1968 May 17;160(3829):774–776. doi: 10.1126/science.160.3829.774. [DOI] [PubMed] [Google Scholar]
  12. Rao P. N., Sunkara P. S., Wilson B. A. Regulation of DNA synthesis: age-dependent cooperation among G1 cells upon fusion. Proc Natl Acad Sci U S A. 1977 Jul;74(7):2869–2873. doi: 10.1073/pnas.74.7.2869. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Rao P. N., Wilson B., Puck T. T. Premature chromosome condensation and cell cycle analysis. J Cell Physiol. 1977 Apr;91(1):131–141. doi: 10.1002/jcp.1040910113. [DOI] [PubMed] [Google Scholar]
  14. Schor S. L., Johnson R. T., Waldren C. A. Changes in the organization of chromosomes during the cell cycle: response to ultraviolet light. J Cell Sci. 1975 May;17(3):539–565. doi: 10.1242/jcs.17.3.539. [DOI] [PubMed] [Google Scholar]
  15. Sperling K., Rao P. N. Mammalian cell fusion. V. Replication behaviour of heterochromatin as observed by premature chromosome condensation. Chromosoma. 1974 Mar 14;45(2):121–131. doi: 10.1007/BF00362306. [DOI] [PubMed] [Google Scholar]
  16. Wolpert-Defilippes M. K., Adamson R. H., Cysyk R. L., Johns D. G. Initial studies on the cytotoxic action of maytansine, a novel ansa macrolide. Biochem Pharmacol. 1975 Mar 15;24(6):751–754. doi: 10.1016/0006-2952(75)90257-9. [DOI] [PubMed] [Google Scholar]

Articles from Proceedings of the National Academy of Sciences of the United States of America are provided here courtesy of National Academy of Sciences

RESOURCES