Table 1.
Thermodynamic data from urea denaturation of BtuB
| Mutant in POPC | ΔG°U (kcal/mol)a | ΔG°U (kcal/mol)b | m (kcal/mol/M) | C0 (M) |
|---|---|---|---|---|
| V10R1 | 2.77 ± 0.21 | 3.01 ± 0.24 | 0.93 ± 0.06 | 3.25 ± 0.13 |
| T30R1 | 3.23 ± 0.11 | 3.33 ± 0.12 | 1.00 ± 0.04 | 3.32 ± 0.04 |
| V78R1 | 2.22 ± 0.02 | 2.12 ± 0.05 | 0.29 ± 0.00 | 7.23 ± 0.13 |
| E108R1 | 2.99 ± 0.11 | 3.04 ± 0.13 | 0.47 ± 0.02 | 6.40 ± 0.14 |
| N128R1 | 2.39 ± 0.05 | 2.53 ± 0.08 | 0.36 ± 0.01 | 6.95 ± 0.12 |
| N151R1 | 2.42 ± 0.09 | 2.63 ± 0.11 | 0.36 ± 0.01 | 7.34 ± 0.14 |
| Q158R1 | 2.89 ± 0.18 | 2.69 ± 0.42 | 0.39 ± 0.06 | 6.91 ± 0.22 |
| W371R1 | 2.90 ± 0.07 | 3.88 ± 0.50 | 0.55 ± 0.07 | 7.05 ± 0.08 |
a
Free energy of unfolding calculated by linear extrapolation (y-intercept).
b
Free energy of unfolding calculated by mC0, where C0 is the midpoint of the unfolding transition calculated by fitting a sigmoidal curve to [urea] vs. peak-to-peak amplitude (see Methods).