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. 1988 Feb 25;16(4):1295–1304. doi: 10.1093/nar/16.4.1295

The histone H3 and H4 mRNAs are polyadenylated in maize.

N Chaubet 1, M E Chaboute 1, B Clément 1, M Ehling 1, G Philipps 1, C Gigot 1
PMCID: PMC336315  PMID: 2831497

Abstract

Northern blot analysis revealed that the histone H3 and H4 mRNAs are of unusual large size in germinating maize embryos. S1-mapping experiments show that the 3'-untranslated regions of the mRNAs transcribed from 3 H3 and 2 H4 maize genes previously described are much longer than in the non-polyadenylated histone mRNAs which represent a major class in animals. Moreover, oligo d(T) cellulose fractionation of RNAs isolated at different developmental stages indicates that more than 99% of the maize H3 and H4 mRNAs are polyadenylated. A putative polyadenylation signal is present in all five genes 17 to 27 nucleotides before the 3'-ends of the mRNAs.

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Selected References

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