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. 1988 Mar 25;16(6):2601–2612. doi: 10.1093/nar/16.6.2601

Randomly picked cosmid clones overlap the pyrB and oriC gap in the physical map of the E. coli chromosome.

V Knott 1, D J Rees 1, Z Cheng 1, G G Brownlee 1
PMCID: PMC336392  PMID: 2834694

Abstract

Sets of overlapping cosmid clones generated by random sampling and fingerprinting methods complement data at pyrB (96.5') and oriC (84') in the published physical map of E. coli. A new cloning strategy using sheared DNA, and a low copy, inducible cosmid vector were used in order to reduce bias in libraries, in conjunction with micro-methods for preparing cosmid DNA from a large number of clones. Our results are relevant to the design of the best approach to the physical mapping of large genomes.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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