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. 1988 Apr 11;16(7):2897–2912. doi: 10.1093/nar/16.7.2897

Intermediates in plasmid pT181 DNA replication.

S Majumder 1, R P Novick 1
PMCID: PMC336440  PMID: 3368310

Abstract

Staphylococcus aureus plasmid pT181 is thought to replicate via an asymmetric rolling-circle mechanism. By studying pulse labeled replicative intermediates, here we report that pT181 replication involves: (1) a post-replicative hypersupercoiled monomer and (2) a partially replicated intermediate which lacks superhelicity but is unlike a typical rolling-circle intermediate in that only nascent strands of less than unit length are released by alkali denaturation. A model for pT181 replication is proposed to accommodate this apparent discrepancy.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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