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. 1988 Apr 11;16(7):2987–3003. doi: 10.1093/nar/16.7.2987

Preparation of covalently linked DNA-RNA hybrids and arabinocytidine containing DNA fragments.

E de Vroom 1, H C Roelen 1, C P Saris 1, T N Budding 1, G A van der Marel 1, J H van Boom 1
PMCID: PMC336446  PMID: 2453027

Abstract

It will be demonstrated that 5'-O-DMT-N-acyl-deoxyribonucleosides, 5'-O-Lev-2'-O-MTHP-N-acyl-ribonucleosides and, also, 2'-O-MTHP-N-acyl-ara-cytidine can be coupled, via the hydroxybenzotriazole phosphotriester approach, to afford two types of DNA-RNA hybrids as well as ara-C containing DNA-fragments. The final removal of acid-labile DMT and MTHP groups could be effected by 1 h treatment with 80% acetic acid of the otherwise unprotected DNA-RNA hybrids. The same acidic hydrolysis did not result in complete removal of the 2'-O-MTHP group from the ara-C unit. Complete deblocking was accomplished after an additional 2 h aqueous HC1 (0.01 M; pH 2.00) treatment.

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Selected References

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