Abstract
A new Type IIS restriction endonuclease was identified, partially purified and characterized from a Bacillus cereus subsp. fluorescens strain. The enzyme recognizes the nonpalindromic sequence ACGGC and cleaves at a distance from it. The cleavage appears to occur with a +/- 1 basepair uncertainty. Thus the cleavage and recognition site is as shown below: ACGGC(N)11-13 TGCCG(N)12-14.
Full text
PDF
Images in this article
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Gayle R. B., 3rd, Auger E. A., Gough G. R., Gilham P. T., Bennett G. N. Formation of MboII vectors and cassettes using asymmetric MboII linkers. Gene. 1987;54(2-3):221–228. doi: 10.1016/0378-1119(87)90490-2. [DOI] [PubMed] [Google Scholar]
- Hasan N., Kim S. C., Podhajska A. J., Szybalski W. A novel multistep method for generating precise unidirectional deletions using BspMI, a class-IIS restriction enzyme. Gene. 1986;50(1-3):55–62. doi: 10.1016/0378-1119(86)90309-4. [DOI] [PubMed] [Google Scholar]
- Kessler C., Höltke H. J. Specificity of restriction endonucleases and methylases--a review. Gene. 1986;47(1):1–153. doi: 10.1016/0378-1119(86)90245-3. [DOI] [PubMed] [Google Scholar]
- Kleid D., Humayun Z., Jeffrey A., Ptashne M. Novel properties of a restriction endonuclease isolated from Haemophilus parahaemolyticus. Proc Natl Acad Sci U S A. 1976 Feb;73(2):293–297. doi: 10.1073/pnas.73.2.293. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Maxam A. M., Gilbert W. Sequencing end-labeled DNA with base-specific chemical cleavages. Methods Enzymol. 1980;65(1):499–560. doi: 10.1016/s0076-6879(80)65059-9. [DOI] [PubMed] [Google Scholar]
- Roberts R. J. Restriction enzymes and their isoschizomers. Nucleic Acids Res. 1987;15 (Suppl):r189–r217. doi: 10.1093/nar/15.suppl.r189. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Shibata T., Ikawa S., Kim C., Ando T. Site-specific deoxyribonucleases in Bacillus subtilis and other Bacillus strains. J Bacteriol. 1976 Oct;128(1):473–476. doi: 10.1128/jb.128.1.473-476.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Szybalski W. Universal restriction endonucleases: designing novel cleavage specificities by combining adapter oligodeoxynucleotide and enzyme moieties. Gene. 1985;40(2-3):169–173. doi: 10.1016/0378-1119(85)90039-3. [DOI] [PubMed] [Google Scholar]
- Vermersch P. S., Bennett G. N. The use of a selectable FokI cassette in DNA replacement mutagenesis of the R388 dihydrofolate reductase gene. Gene. 1987;54(2-3):229–238. doi: 10.1016/0378-1119(87)90491-4. [DOI] [PubMed] [Google Scholar]