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. 1988 Apr 25;16(8):3255–3268. doi: 10.1093/nar/16.8.3255

Adenosine deaminase gene expression is regulated posttranscriptionally in the nucleus.

T M Berkvens 1, F Schoute 1, H van Ormondt 1, P Meera Khan 1, A J van der Eb 1
PMCID: PMC336492  PMID: 3163799

Abstract

The housekeeping enzyme adenosine deaminase (ADA) shows a large variation in tissue-specific expression ranging from 1 Iu in red blood cells to 880 Iu in thymocytes. We investigated the acute lymphocytic leukemic cell line Molt-4 (660 Iu ADA/g protein) and the promyelocytic cell line HL-60 (38 Iu ADA/g protein) as a model system to determine the levels at which the tissue-specific expression of ADA is regulated. From our results it can be concluded that the almost 20-fold difference in ADA expression between Molt-4 and HL-60 is the result of differences in the post-transcriptional processing and/or stability of ADA pre-mRNA within the nucleus.

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Selected References

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