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. 1988 May 25;16(10):4447–4463. doi: 10.1093/nar/16.10.4447

Cloning and characterization of a photolyase gene from the cyanobacterium Anacystis nidulans.

A Yasui 1, M Takao 1, A Oikawa 1, A Kiener 1, C T Walsh 1, A P Eker 1
PMCID: PMC336641  PMID: 2837735

Abstract

A 2 kb fragment was isolated from an Anacystis nidulans genomic DNA library by hybridization with synthetic oligonucleotide probes derived from the N-terminal amino acid sequence of Anacystis photolyase. This fragment contains a 1452 bp-long open reading frame encoding a polypeptide of 484 amino acids (Mr 54475). Antibodies raised against purified Anacystis photolyase reacted with extracts of cells harboring fused genes between lacZ of Escherichia coli and this gene. A 40.7% similarity was found between the deduced amino acid sequences of Anacystis and E. coli photolyases, notwithstanding the difference in chromophore structure.

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Selected References

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