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. 1988 May 25;16(10):4511–4524. doi: 10.1093/nar/16.10.4511

Bacteriophage T7 late promoters with point mutations: quantitative footprinting and in vivo expression.

K A Chapman 1, S I Gunderson 1, M Anello 1, R D Wells 1, R R Burgess 1
PMCID: PMC336645  PMID: 3288970

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Selected References

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  1. Birnboim H. C., Doly J. A rapid alkaline extraction procedure for screening recombinant plasmid DNA. Nucleic Acids Res. 1979 Nov 24;7(6):1513–1523. doi: 10.1093/nar/7.6.1513. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Chakraborty P. R., Sarkar P., Huang H. H., Maitra U. Studies on T3-induced ribonucleic acid polymerase. 3. Purification and characterization of the T3-induced ribonucleic acid polymerase from bacteriophage T3-infected Escherichia coli cells. J Biol Chem. 1973 Oct 10;248(19):6637–6646. [PubMed] [Google Scholar]
  3. Chapman K. A., Burgess R. R. Construction of bacteriophage T7 late promoters with point mutations and characterization by in vitro transcription properties. Nucleic Acids Res. 1987 Jul 10;15(13):5413–5432. doi: 10.1093/nar/15.13.5413. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Davanloo P., Rosenberg A. H., Dunn J. J., Studier F. W. Cloning and expression of the gene for bacteriophage T7 RNA polymerase. Proc Natl Acad Sci U S A. 1984 Apr;81(7):2035–2039. doi: 10.1073/pnas.81.7.2035. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Dunn J. J., Studier F. W. Complete nucleotide sequence of bacteriophage T7 DNA and the locations of T7 genetic elements. J Mol Biol. 1983 Jun 5;166(4):477–535. doi: 10.1016/s0022-2836(83)80282-4. [DOI] [PubMed] [Google Scholar]
  6. Godson G. N., Vapnek D. A simple method of preparing large amounts of phiX174 RF 1 supercoiled DNA. Biochim Biophys Acta. 1973 Apr 11;299(4):516–520. doi: 10.1016/0005-2787(73)90223-2. [DOI] [PubMed] [Google Scholar]
  7. Gross C. A., Grossman A. D., Liebke H., Walter W., Burgess R. R. Effects of the mutant sigma allele rpoD800 on the synthesis of specific macromolecular components of the Escherichia coli K12 cell. J Mol Biol. 1984 Jan 25;172(3):283–300. doi: 10.1016/s0022-2836(84)80027-3. [DOI] [PubMed] [Google Scholar]
  8. Ikeda R. A., Richardson C. C. Interactions of the RNA polymerase of bacteriophage T7 with its promoter during binding and initiation of transcription. Proc Natl Acad Sci U S A. 1986 Jun;83(11):3614–3618. doi: 10.1073/pnas.83.11.3614. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Maxam A. M., Gilbert W. Sequencing end-labeled DNA with base-specific chemical cleavages. Methods Enzymol. 1980;65(1):499–560. doi: 10.1016/s0076-6879(80)65059-9. [DOI] [PubMed] [Google Scholar]
  10. Osterman H. L., Coleman J. E. T7 ribonucleic acid polymerase-promotor interactions. Biochemistry. 1981 Aug 18;20(17):4884–4892. doi: 10.1021/bi00520a013. [DOI] [PubMed] [Google Scholar]
  11. Panayotatos N., Wells R. D. Recognition and initiation site for four late promoters of phage T7 is a 22-base pair DNA sequence. Nature. 1979 Jul 5;280(5717):35–39. doi: 10.1038/280035a0. [DOI] [PubMed] [Google Scholar]
  12. Rosa M. D. Four T7 RNA polymerase promoters contain an identical 23 bp sequence. Cell. 1979 Apr;16(4):815–825. doi: 10.1016/0092-8674(79)90097-7. [DOI] [PubMed] [Google Scholar]
  13. Ryan M. J., Wells R. D. Coumerimycin A1: A preferential inhibitor of replicative DNA synthesis in Escherichia coli. II. In vivo characterization. Biochemistry. 1976 Aug 24;15(17):3778–3782. doi: 10.1021/bi00662a021. [DOI] [PubMed] [Google Scholar]
  14. Strothkamp R. E., Oakley J. L., Coleman J. E. Promoter melting by T7 ribonucleic acid polymerase as detected by single-stranded endonuclease digestion. Biochemistry. 1980 Mar 18;19(6):1074–1080. doi: 10.1021/bi00547a005. [DOI] [PubMed] [Google Scholar]
  15. Tabor S., Richardson C. C. A bacteriophage T7 RNA polymerase/promoter system for controlled exclusive expression of specific genes. Proc Natl Acad Sci U S A. 1985 Feb;82(4):1074–1078. doi: 10.1073/pnas.82.4.1074. [DOI] [PMC free article] [PubMed] [Google Scholar]

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