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. 1988 May 25;16(10):4637–4650. doi: 10.1093/nar/16.10.4637

The thermal stability of oligonucleotide duplexes is sequence independent in tetraalkylammonium salt solutions: application to identifying recombinant DNA clones.

K A Jacobs 1, R Rudersdorf 1, S D Neill 1, J P Dougherty 1, E L Brown 1, E F Fritsch 1
PMCID: PMC336654  PMID: 3380691

Abstract

In solutions of tetraalkylammonium salts the melting temperature of oligonucleotide duplexes is independent of nucleotide sequence and thus GC content. Data quantitating the destabilizing effects of various mismatches in these solvents are also presented. The results are in accord with theories on DNA melting and establish conditions under which oligonucleotides can be used as hybridization probes with predictable and controllable specificity.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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